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Product Info Summary
| SKU: | A06546-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-LSM14A Antibody Picoband®
SKU/Catalog Number
A06546-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LSM14A Antibody Picoband® catalog # A06546-1. Tested in WB, IP, IF, ICC, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LSM14A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06546-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human LSM14A recombinant protein (Position: F105-R404).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06546-1 is reactive to LSM14A in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
51 kDa
Background of LSM14A
Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06546-1 is guaranteed for ELISA, Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HEL whole cell, human A549 whole cell, human SH-SY5Y whole cell, human A431 whole cell
IF: Hela cells
FCM: SH-SY5Y cells
IP: A549 cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LSM14A using anti-LSM14A antibody (A06546-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSM14A antigen affinity purified polyclonal antibody (Catalog # A06546-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSM14A at approximately 60 kDa. The expected band size for LSM14A is at 51 kDa.
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Figure 2. IF analysis of LSM14A using anti-LSM14A antibody (A06546-1).
LSM14A was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-LSM14A Antibody (A06546-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of SH-SY5Y cells using anti-LSM14A antibody (A06546-1).
Overlay histogram showing SH-SY5Y cells stained with A06546-1(Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LSM14A Antibody (A06546-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 4. Immunoprecipitating LSM14A in A549 whole cell lysate .
Western blot analysis of LSM14A using anti-LSM14A antibody (A06546-1).
Lane 1: A549 whole cell lysates (30ug)
Lane 2: Rabbit control IgG instead of anti-LSM14A antibody in A549 whole cell lysate.
Lane 3: anti-LSM14A antibody (2μg) + A549 whole cell lysate (500μg)
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LSM14A antigen affinity purified polyclonal antibody (A06546-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for LSM14A at approximately 60 kDa. The expected band size for LSM14A is at 51 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LSM14A (Source: Uniprot.org, NCBI)
Gene Name
LSM14A
Full Name
Protein LSM14 homolog A
Weight
51 kDa
Superfamily
LSM14 family
Alternative Names
alphaSNBP; C19orf13; DKFZP434D1335; FAM61A; family with sequence similarity 61, member A; hRAP55; hRAP55A; LSM14 homolog A; LSM14A, SCD6 homolog A (S. cerevisiae); Protein FAM61A; protein LSM14 homolog A; Protein SCD6 homolog; Putative alpha-synuclein-binding protein; RAP55ALSM14 homolog A (SCD6, S. cerevisiae); RAP55chromosome 19 open reading frame 13; RNA-associated protein 55; RNA-associated protein 55A LSM14A C19orf13, FAM61A, RAP55, RAP55A LSM14A mRNA processing body assembly factor protein LSM14 homolog A|LSM14 homolog A|LSM14A, SCD6 homolog A|RNA-associated protein 55|RNA-associated protein 55A|alphaSNBP|family with sequence similarity 61, member A|hRAP55|hRAP55A|protein SCD6 homolog|putative alpha-synuclein-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LSM14A, check out the LSM14A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LSM14A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LSM14A Antibody Picoband® (A06546-1)
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