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Product Info Summary
| SKU: | A08734 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-LRRFIP2 Antibody
SKU/Catalog Number
A08734
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LRRFIP2 Antibody (Catalog # A08734). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
LRRFIP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-LRRFIP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08734)
Host
Rabbit
Contents
LRRFIP2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LRRFIP2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human LRRFIP2. The immunogen is located within amino acids 620 - 670 of LRRFIP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Three isoforms of LRRFIP2 are known to exist; this antibody will recognize all three. LRRFIP2 antibody is predicted to not cross-react with other LRRFIP family members.
Reactive Species
A08734 is reactive to LRRFIP2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
82171 MW
Background of LRRFIP2
The leucine-rich repeat FLI-I-interacting protein 2 (LRRFIP2), like the related protein LRRFIP1, was identified in a yeast two-hybrid system through binding to the LRR domain of human FLI. It can activate the Wnt signaling pathway in cultured cells and is thought to be a component of the Wnt signaling pathway that modulates Wnt signaling through interactions with Disheveled to increase the cellular levels and transcription activity of beta-catenin. LRRFIP2 has recently been characterized as a positive regulator of the TLR4 signaling pathway for activating NF-κB during the early host response to LPS stimulation through binding to the TLR adaptor protein MyD88, and that this interaction with MyD88 is governed by phosphorylation of specific residues in LRRFIP2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08734 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LRRFIP2 antibody can be used for detection of LRRFIP2 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LRRFIP2 in rat colon tissue lysate with LRRFIP2 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of LRRFIP2 in human colon tissue with LRRFIP2 antibody at 5 μg/mL.
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Immunofluorescence of LRRFIP2 in human colon tissue with LRRFIP2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For LRRFIP2 (Source: Uniprot.org, NCBI)
Gene Name
LRRFIP2
Full Name
Leucine-rich repeat flightless-interacting protein 2
Weight
82171 MW
Superfamily
LRRFIP family
Alternative Names
HUFI-2, Leucine-rich repeat flightless-interacting protein 2, LRR FLII-interacting protein 2 LRRFIP2 HUFI-2 LRR binding FLII interacting protein 2 leucine-rich repeat flightless-interacting protein 2|LRR FLII-interacting protein 2|leucine rich repeat (in FLII) interacting protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LRRFIP2, check out the LRRFIP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LRRFIP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LRRFIP2 Antibody (A08734)
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