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Product Info Summary
| SKU: | A06721-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-LPAR5 Antibody Picoband®
View all LPAR5/LPA5/GPR92 Antibodies
SKU/Catalog Number
A06721-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-LPAR5 Antibody Picoband® catalog # A06721-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LPAR5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06721-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human LPAR5 recombinant protein (Position: A83-L372). Human LPAR5 shares 77.9% amino acid (aa) sequence identity with mouse LPAR5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06721-1 is reactive to LPAR5 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
41.347kDa
Background of LPAR5/LPA5/GPR92
Lysophosphatidic acid receptor 5 also known as LPA5 is a protein that in humans is encoded by the LPAR5 gene. This gene encodes a member of the rhodopsin class of G protein-coupled transmembrane receptors. This protein transmits extracellular signals from lysophosphatidic acid to cells through heterotrimeric G proteins and mediates numerous cellu
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06721-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HEL whole cell, human K562 whole cell, human A431 whole cell, human Hacat whole cell
FCM: K562 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GPR92/LPAR5 using anti-GPR92/LPAR5 antibody (A06721-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human Hacat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPR92/LPAR5 antigen affinity purified polyclonal antibody (Catalog # A06721-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPR92/LPAR5 at approximately 41 kDa. The expected band size for GPR92/LPAR5 is at 41 kDa.
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Figure 2. Flow Cytometry analysis of K562 cells using anti-GPR92/LPAR5 antibody (A06721-1).
Overlay histogram showing K562 cells stained with A06721-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GPR92/LPAR5 Antibody (A06721-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For LPAR5 (Source: Uniprot.org, NCBI)
Gene Name
LPAR5
Full Name
Lysophosphatidic acid receptor 5
Weight
41.347kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
G protein-coupled receptor 92; GPR92; GPR92G-protein coupled receptor 92; GPR93; GPR93G-protein coupled receptor 93; KPG_010; LPA receptor 5; LPA5; LPA5LPA-5; LPAR5; lysophosphatidic acid receptor 5 LPAR5 GPR92, GPR93, KPG_010, LPA5 lysophosphatidic acid receptor 5 lysophosphatidic acid receptor 5|G protein-coupled receptor 92|G-protein coupled receptor 93|LPA receptor 5|LPA-5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LPAR5, check out the LPAR5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LPAR5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LPAR5 Antibody Picoband® (A06721-1)
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