Anti-LPAR3 Antibody Picoband™

Product Name

Anti-LPAR3 Antibody Picoband™

View all LPAR3/LPA3/EDG-7 Antibodies

SKU/Catalog Number

A06597-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-LPAR3 Antibody Picoband™ catalog # A06597-3. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-LPAR3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06597-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human LPAR3 recombinant protein (Position: M1-S353).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A06597-3 is reactive to LPAR3 in Human, Mouse, Rat

Applications

A06597-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

52588 MW

Background of LPAR3/LPA3/EDG-7

Lysophosphatidic acid receptor 3 also known as LPA3 is a protein that in humans is encoded by the LPAR3 gene. This gene encodes a member of the G protein-coupled receptor family, as well as the EDG family of proteins. This protein functions as a cellular receptor for lysophosphatidic acid and mediates lysophosphatidic acid-evoked calcium mobilization. This receptor couples predominantly to G(q/11) alpha proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of EDG7/LPAR3 using anti-EDG7/LPAR3 antibody (A06597-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates,
Lane 3: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG7/LPAR3 antigen affinity purified polyclonal antibody (Catalog # A06597-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDG7/LPAR3 at approximately 43 kDa. The expected band size for EDG7/LPAR3 is at 40 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of RT4 cells using anti-EDG7/LPAR3 antibody (A06597-3).
Overlay histogram showing RT4 cells stained with A06597-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-EDG7/LPAR3 Antibody (A06597-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For LPAR3 (Source: Uniprot.org, NCBI)

Alternative Names

EDG7; Edg-7; EDG7FLJ98231; GPCR; HOFNH30; LPA-3; LPA3LPA receptor 3; LPAR3; lysophosphatidic acid G-protein-coupled receptor7,LPA receptor EDG7; lysophosphatidic acid receptor 3; Lysophosphatidic acid receptor Edg-7; RP4-678I3 LPAR3 EDG7, Edg-7, GPCR, HOFNH30, LP-A3, LPA3, RP4-678I3 lysophosphatidic acid receptor 3 lysophosphatidic acid receptor 3|LPA receptor 3|LPA receptor EDG7|LPA-3|calcium-mobilizing lysophosphatidic acid receptor LP-A3|endothelial cell differentiation gene 7|endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7|lysophosphatidic acid receptor Edg-7

For more info on LPAR3, check out the LPAR3 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LPAR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the LPAR3 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis