Click image to see more details
Product Info Summary
| SKU: | A05290-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Loricrin/LOR Antibody Picoband®
SKU/Catalog Number
A05290-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Loricrin/LOR Antibody Picoband® catalog # A05290-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Loricrin/LOR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05290-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Loricrin/LOR recombinant protein (Position: M1-K312).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05290-1 is reactive to LOR in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
39411 MW
Background of LORICRIN
Loricrin is a protein that in humans is encoded by the LOR gene. It is mapped to 1q21.3. Loricrin is a major protein component of the cornified cell envelope found in terminally differentiatedepidermal cells. It is expressed in the granular layer of all keratinized epithelial cells of mammals tested including oral, esophageal and stomach mucosa of rodents, tracheal squamous metaplasia of vitamin A deficient hamster and estrogen induced squamous vaginal epithelium of rats.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05290-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human U-87MG whole cell, human K562 whole cell, human U-937 whole cell, human THP-1 whole cell, rat heart tissue, rat small intestine tissue, mouse heart tissue, mouse small intestine tissue, mouse Neuro-2a whole cell,
IHC: human sarcoma tissue, rat small intestine tissue, mouse small intestine tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LOR using anti-LOR antibody (A05290-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates
Lane 2: human K562 whole cell lysates
Lane 3: human U-937 whole cell lysates
Lane 4: human THP-1 whole cell lysates
Lane 5: rat heart tissue lysates
Lane 6: rat small intestine tissue lysates
Lane 7: mouse heart tissue lysates
Lane 8: mouse small intestine tissue lysates
Lane 9: mouse Neuro-2a whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOR antigen affinity purified polyclonal antibody (Catalog # A05290-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOR at approximately 26KD. The expected band size for LOR is at 26KD.
Click image to see more details
Figure 2. IHC analysis of LOR using anti-LOR antibody (A05290-1).
LOR was detected in paraffin-embedded section of human sarcoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LOR Antibody (A05290-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of LOR using anti-LOR antibody (A05290-1).
LOR was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LOR Antibody (A05290-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of LOR using anti-LOR antibody (A05290-1).
LOR was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LOR Antibody (A05290-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For LOR (Source: Uniprot.org, NCBI)
Gene Name
LOR
Full Name
Loricrin
Weight
39411 MW
Alternative Names
Loricrin; LOR; LRN LORICRIN LOR loricrin cornified envelope precursor protein loricrin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LOR, check out the LOR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LOR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Loricrin/LOR Antibody Picoband® (A05290-1)
Hello CJ!
A05290-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lee CW,Su YH,Chiang YC,Lee IT,Li SY,Lee HC,Hsu LF,Yan YL,Li HY,Chen MC,Peng KT,Lai CH. Glycofullerenes Inhibit Particulate Matter Induced Inflammation and Loss of Barrier Proteins in HaCaT Human Keratinocytes. Biomolecules.2020 Mar 28;10(4):514.doi:10.339
Species: Human
A05290-1 usage in article: APP:WB, SAMPLE:HACAT CELL, DILUTION:NA
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Loricrin/LOR Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Loricrin/LOR Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-Loricrin/LOR Antibody Picoband®
Question
Is a blocking peptide available for product anti-Loricrin/LOR antibody (A05290-1)?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
We do provide the blocking peptide for product anti-Loricrin/LOR antibody (A05290-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-24
Question
Does anti-Loricrin/LOR antibody A05290-1 work on dog IHC-P with skin?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
Our lab technicians have not tested anti-Loricrin/LOR antibody A05290-1 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Loricrin/LOR antibody A05290-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog skin in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-10
Question
We are currently using anti-Loricrin/LOR antibody A05290-1 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
The anti-Loricrin/LOR antibody (A05290-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-09
Question
Do you have a BSA free version of anti-Loricrin/LOR antibody A05290-1 available?
Verified Customer
Verified customer
Asked: 2018-09-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Loricrin/LOR antibody A05290-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-07
Question
Would anti-Loricrin/LOR antibody A05290-1 work for IHC-P with upper arm skin?
S. Kulkarni
Verified customer
Asked: 2017-04-11
Answer
According to the expression profile of upper arm skin, LOR is highly expressed in upper arm skin. So, it is likely that anti-Loricrin/LOR antibody A05290-1 will work for IHC-P with upper arm skin.
Boster Scientific Support
Answered: 2017-04-11
Question
I would like to test anti-Loricrin/LOR antibody A05290-1 on human upper arm skin for research purposes, then I may be interested in using anti-Loricrin/LOR antibody A05290-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Singh
Verified customer
Asked: 2014-11-27
Answer
The products we sell, including anti-Loricrin/LOR antibody A05290-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-11-27
Question
Can you help my question with product A05290-1, anti-Loricrin/LOR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Miller
Verified customer
Asked: 2013-07-29
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05290-1 anti-Loricrin/LOR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-07-29
