Anti-LMBRD1 Antibody

Product Name

Anti-LMBRD1 Antibody

View all LMBRD1 Antibodies

SKU/Catalog Number

A08163

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LMBRD1 Antibody (Catalog # A08163). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

LMBRD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-LMBRD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08163)

Host

Rabbit

Contents

LMBRD1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

LMBRD1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human LMBRD1. The immunogen is located within the last 50 amino acids of LMBRD1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least four isoforms of ZBTB4 are known to exist; this antibody will only recognize the longest isoform. This antibody is predicted to not cross-react with other ZBTB protein family members.

Reactive Species

A08163 is reactive to LMBRD1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

61389 MW

Background of LMBRD1

LMBRD1, also known as NESI (nuclear export signal-interacting protein, is a lysosomal membrane protein that is thought be involved in the transport and metabolism of cobalamin. LMBRD1 was initially identified as interacting with the large form of the hepatitis delta antigen and may be required for the nucleocytoplasmic shuttling of the hepatitis delta virus. Mutations in this gene are associated with the vitamin B12 metabolism disorder termed, homocystinuria-megaloblastic anemia complementation type F (cblF).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08163 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

LMBRD1 antibody can be used for detection of LMBRD1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For LMBRD1 (Source: Uniprot.org, NCBI)

Alternative Names

NESI, LMBD1, MAHCF, C6orf209, NESI, BM-021, CD001, MSTP044, Probable lysosomal cobalamin transporter, HDAg-L-interacting protein NESI LMBRD1 C6orf209, LMBD1, MAHCF, NESI LMBR1 domain containing 1 lysosomal cobalamin transport escort protein LMBD1|HDAg-L-interacting protein NESI|hepatitis delta -L interacting protein|liver regeneration p-53 related protein|nuclear export signal-interacting protein|probable lysosomal cobalamin transporter

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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