Anti-LLGL2 Antibody Picoband™

Product Name

Anti-LLGL2 Antibody Picoband™

View all LLGL2 Antibodies

SKU/Catalog Number

A08503-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-LLGL2 Antibody Picoband™ catalog # A08503-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-LLGL2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08503-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human LLGL2 recombinant protein (Position: L102-Q553). Human LLGL2 shares 91.2% amino acid (aa) sequence identity with mouse LLGL2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08503-1 is reactive to LLGL2 in Human

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

120 kDa

Calculated molecular weight

Background of LLGL2

The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division, epithelial cell polarity, and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08503-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Positive Control

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of LLGL2 using anti-LLGL2 antibody (A08503-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LLGL2 antigen affinity purified polyclonal antibody (Catalog # A08503-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LLGL2 at approximately 120 kDa. The expected band size for LLGL2 is at 113 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-LLGL2 antibody (A08503-1).
Overlay histogram showing HepG2 cells stained with A08503-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LLGL2 Antibody (A08503-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For LLGL2 (Source: Uniprot.org, NCBI)

Alternative Names

HGLlethal giant larvae (Drosophila) homolog 2; human giant larvae homolog; lethal giant larvae homolog 2 (Drosophila); lethal(2) giant larvae protein homolog 2; LGL2 LLGL2 HGL, Hugl-2, LGL2 LLGL scribble cell polarity complex component 2 LLGL scribble cell polarity complex component 2|LLGL2, scribble cell polarity complex component|human giant larvae homolog|lethal giant larvae homolog 2, scribble cell polarity complex component|lethal(2) giant larvae protein homolog 2

For more info on LLGL2, check out the LLGL2 Infographic

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In this infographic, you will see the following information for LLGL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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