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Product Info Summary
| SKU: | A04072-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LIMS1 Antibody Picoband®
View all PINCH1/LIMS1 Antibodies
SKU/Catalog Number
A04072-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-LIMS1 Antibody Picoband® catalog # A04072-2. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LIMS1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04072-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human LIMS1 recombinant protein (Position: E11-A172). Human LIMS1 shares 100% amino acid (aa) sequence identity with mouse LIMS1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04072-2 is reactive to LIMS1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
37.251kDa
Background of PINCH1/LIMS1
LIM and senescent cell antigen-like-containing domain protein 1 is a protein that in humans is encoded by the LIMS1 gene. The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04072-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Caco-2 whole cell, human Hela whole cell, human 293T whole cell, human Jurkat whole cell, rat lung tissue, rat kidney tissue, mouse lung tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LIMS1 using anti-LIMS1 antibody (A04072-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIMS1 antigen affinity purified polyclonal antibody (Catalog # A04072-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIMS1 at approximately 37 kDa. The expected band size for LIMS1 is at 37 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LIMS1 (Source: Uniprot.org, NCBI)
Gene Name
LIMS1
Full Name
LIM and senescent cell antigen-like-containing domain protein 1
Weight
37.251kDa
Alternative Names
LIM and senescent cell antigen-like domains 1; LIM and senescent cell antigen-like-containing domain protein 1; LIMS1; PINCH1; PINCH-1; PINCHPINCH1Particularly interesting new Cys-His protein 1; Renal carcinoma antigen NY-REN-48 LIMS1 PINCH, PINCH-1, PINCH1 LIM zinc finger domain containing 1 LIM and senescent cell -like-containing domain protein 1|LIM and senescent cell -like domains 1|LIM-type zinc finger domains 1|particularly interesting new Cys-His protein 1|renal carcinoma NY-REN-48|senescent cell
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LIMS1, check out the LIMS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LIMS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LIMS1 Antibody Picoband® (A04072-2)
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