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Product Info Summary
| SKU: | M00239 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-LGR5/GPR49 Rabbit Monoclonal Antibody
View all Lgr5/GPR49 Antibodies
SKU/Catalog Number
M00239
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LGR5/GPR49 Rabbit Monoclonal Antibody catalog # M00239. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LGR5/GPR49 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00239)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CHF-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LGR5/GPR49
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00239 is reactive to LGR5 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100-110 kDa
Calculated molecular weight
99998 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00239 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
FC 1:200
Positive Control
WB: Human fetal skeletal muscle
Validation Images & Assay Conditions
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Western blot analysis of GPR49 expression in Human fetal skeletal muscle lysate.
Protein Target Info & Infographic
Gene/Protein Information For LGR5 (Source: Uniprot.org, NCBI)
Gene Name
LGR5
Full Name
Leucine-rich repeat-containing G-protein coupled receptor 5
Weight
99998 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Leucine-rich repeat-containing G-protein coupled receptor 5;G-protein coupled receptor 49;G-protein coupled receptor 67;G-protein coupled receptor HG38;LGR5;GPR49, GPR67; LGR5 FEX, GPR49, GPR67, GRP49, HG38 leucine rich repeat containing G protein-coupled receptor 5 leucine-rich repeat-containing G-protein coupled receptor 5|G-protein coupled receptor 49|G-protein coupled receptor 67|G-protein coupled receptor HG38|orphan G protein-coupled receptor HG38
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LGR5, check out the LGR5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LGR5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LGR5/GPR49 Rabbit Monoclonal Antibody (M00239)
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No publications found for M00239
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Customer Q&As
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1 Customer Q&As for Anti-LGR5/GPR49 Rabbit Monoclonal Antibody
Question
We are currently using anti-LGR5/GPR49 Rabbit Monoclonal antibody M00239 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
J. Jackson
Verified customer
Asked: 2017-01-05
Answer
The anti-LGR5/GPR49 Rabbit Monoclonal antibody (M00239) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-01-05
