Anti-LGI3 Antibody

Product Name

Anti-LGI3 Antibody

View all LGI3 Antibodies

SKU/Catalog Number

A10809

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LGI3 Antibody (Catalog # A10809). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

LGI3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-LGI3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10809)

Host

Rabbit

Contents

LGI3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

LGI3 antibody was raised against a 12 amino acid synthetic peptide from near the center of human LGI3. The immunogen is located within amino acids 390 - 440 of LGI3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Two isoforms of LGI3 are known to exist; this LGI3 antibody will recognize both. This LGI3 antibody is predicted to be specific to LGI3 and not recognize other LGI proteins. The observed higher MW band may represent a PTM form of LGI3.

Reactive Species

A10809 is reactive to LGI3 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

61704 MW

Background of LGI3

The leucine-rich, glioma inactivated gene 3 (LGI3) is a member of the LGI family in which LGI1 is the exemplar. The LGI family consists of four of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 has been identified as a candidate tumor suppressor gene for glioma and plays a role in autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Despite its high homology with LGI1 and similar pattern of expression, mutations in LGI3 have not been found to be associated with ADTLE. LGI3 expression is induced in rat astrocyte cultures by the amyloid beta (Abeta) peptide and accumulated on neuronal plasma membranes of aged monkey brains and co-localized with Abeta.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10809 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

LGI3 antibody can be used for the detection of LGI3 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For LGI3 (Source: Uniprot.org, NCBI)

Alternative Names

LGIL4, LGIL4, UNQ8190/PRO23199, Leucine-rich repeat LGI family member 3, LGI1-like protein 4 LGI3 LGIL4 leucine rich repeat LGI family member 3 leucine-rich repeat LGI family member 3|LGI1-like protein 4|leucine-rich glioma-inactivated protein 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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