Click image to see more details
Product Info Summary
| SKU: | M04194 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04194
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody catalog # M04194. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04194)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOAB-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LAMA3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04194 is reactive to LAMA3 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
367 kDa
Calculated molecular weight
366649 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04194 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of LAMA3 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For LAMA3 (Source: Uniprot.org, NCBI)
Gene Name
LAMA3
Full Name
Laminin subunit alpha-3
Weight
366649 MW
Alternative Names
Laminin subunit alpha-3;Epiligrin 170 kDa subunit;E170;Epiligrin subunit alpha;Kalinin subunit alpha;Laminin-5 subunit alpha;Laminin-6 subunit alpha;Laminin-7 subunit alpha;Nicein subunit alpha;LAMA3;LAMNA; LAMA3 BM600, E170, LAMNA, LOCS laminin subunit alpha 3 laminin subunit alpha-3|BM600 150kD subunit|LAM3, alpha-3 subunit|epiligrin 170 kda subunit|epiligrin alpha 3 subunit|kalinin 165kD subunit|kalinin subunit alpha|laminin 5, alpha-3 subunit|laminin A3|laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin)|laminin-5 alpha 3 chain|laminin-5 subunit alpha|laminin-6 subunit alpha|laminin-7 subunit alpha|nicein 150kD subunit|nicein subunit alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LAMA3, check out the LAMA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LAMA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody (M04194)
Hello CJ!
No publications found for M04194
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody
Question
We are currently using anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody M04194 for human tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
The anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody (M04194) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-24
Question
Is this M04194 anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody reactive to the isotypes of LAMA3?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
The immunogen of M04194 anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody is A synthesized peptide derived from human LAMA3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-25
Question
I was wanting to use your anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody for IF for human ectocervix on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ectocervix identification?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
It shows on the product datasheet, M04194 anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody has been tested for IF, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human ectocervix in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-07
Question
I am interested in to test anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody M04194 on human ectocervix for research purposes, then I may be interested in using anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody M04194 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-22
Answer
The products we sell, including anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody M04194, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-22
Question
Is a blocking peptide available for product anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody (M04194)?
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
We do provide the blocking peptide for product anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody (M04194). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-12
Question
I have a question about product M04194, anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
H. Bhatt
Verified customer
Asked: 2017-05-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04194 anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-09
