Anti-Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Monoclonal Antibody

Product Name

Anti-Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Monoclonal Antibody

View all Cadherin-16 Antibodies

SKU/Catalog Number

M07518

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Monoclonal Antibody (Catalog # M07518). Tested in Flow Cytometry, WB, IHC applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Dog.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07518)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: rCDH16/1071

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human CDH16 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M07518 is reactive to CDH16 in Dog, Human, Mouse, Rabbit, Rat

Applications

M07518 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

89923 MW

Background of Cadherin-16

This monoclonal antibody recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Anti-KSP-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH16/1071).

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Western Blot analysis of human kidney tissue lysate using Anti-KSP-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH16/1071).

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Flow Cytometric analysis of Human HEK293 cells using Anti-KSP-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH16/1071) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Gene/Protein Information For CDH16 (Source: Uniprot.org, NCBI)

Alternative Names

cadherin 16, KSP-cadherin; cadherin-16; Kidney-specific cadherin; KSP-cadherin CDH16 cadherin 16 cadherin-16|KSP-cadherin|cadherin 16, KSP-cadherin|kidney-specific cadherin

For more info on CDH16, check out the CDH16 Infographic

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In this infographic, you will see the following information for CDH16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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