Anti-KRIT1 Antibody Picoband®

Product Name

Anti-KRIT1 Antibody Picoband®

View all KRIT1 Antibodies

SKU/Catalog Number

PB10074

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-KRIT1 Antibody Picoband® catalog # PB10074. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KRIT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10074)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human KRIT1, different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10074 is reactive to KRIT1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

84 kDa

Calculated molecular weight

84348 MW

Background of KRIT1

Krev interaction trapped protein 1 (KRIT1) is a protein that in humans is encoded by the CCM1 gene. This gene encodes a protein containing four ankyrin repeats, a band 4.1/ezrin/radixin/moesin (FERM) domain, and multiple NPXY sequences. The encoded protein is localized in the nucleus and cytoplasm. It binds to integrin cytoplasmic domain-associated protein-1 alpha (ICAP1alpha), and plays a critical role in beta1-integrin-mediated cell proliferation. It associates with junction proteins and RAS-related protein 1A (Rap1A), which requires the encoded protein for maintaining the integrity of endothelial junctions. It is also a microtubule-associated protein and may play a role in microtubule targeting. Mutations in this gene result in cerebral cavernous malformations. Multiple alternatively spliced transcript variants have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB10074 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: rat cardiac muscle tissue, NIH3T3 whole cell, HELA whole cell

Validation Images & Assay Conditions

Gene/Protein Information For KRIT1 (Source: Uniprot.org, NCBI)

Alternative Names

Krev interaction trapped protein 1;Krev interaction trapped 1;Cerebral cavernous malformations 1 protein;KRIT1;CCM1; KRIT1 CAM, CCM1 KRIT1 ankyrin repeat containing krev interaction trapped protein 1|ankyrin repeat-containing protein Krit1|cerebral cavernous malformations 1 protein|krev interaction trapped 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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