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Product Info Summary
| SKU: | A02616 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-KLOTHO Antibody
SKU/Catalog Number
A02616
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-KLOTHO Antibody (Catalog # A02616). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
KLOTHO antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-KLOTHO Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02616)
Host
Rabbit
Contents
KLOTHO Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-KLOTHO antibody was raised against a peptide corresponding to 17 amino acids near the center of human KLOTHO. The immunogen is located within amino acids 410-460 of KLOTHO.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Three isoforms of KLOTHO are known to exist.
Reactive Species
A02616 is reactive to KL in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
116181 MW
Background of Klotho
KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02616 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-4 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: KLOTHO A02616 (2 μg/mL), KLOTHO, competitor antibody (4 μg/mL) and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation with Recombinant Protein
Loading: 30 ng of human KLOTHO recombinant protein per lane.Antibodies: KLOTHO A02616 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 72kD.
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Western Blot Validation in Rat Kidney Tissue Lysate
Loading: 15 μg of lysates per lane. Antibodies: KLOTHO A02616 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of KLOTHO in Mouse Heart Tissue
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-KLOTHO antibody (A02616) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of KLOTHO in Mouse Heart Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling KLOTHO with A02616 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Immunohistochemistry Validation of KLOTHO in Rat Heart Tissue
Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-KLOTHO antibody (A02616) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Protein Target Info & Infographic
Gene/Protein Information For KL (Source: Uniprot.org, NCBI)
Gene Name
KL
Full Name
Klotho
Weight
116181 MW
Superfamily
glycosyl hydrolase 1 family
Alternative Names
Klotho KL HFTC3 klotho klotho
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KL, check out the KL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KLOTHO Antibody (A02616)
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