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Product Info Summary
| SKU: | A02951-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-KIF1B Antibody Picoband®
SKU/Catalog Number
A02951-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KIF1B Antibody Picoband® catalog # A02951-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-KIF1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02951-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human KIF1B recombinant protein (Position: D390-L1314).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02951-1 is reactive to KIF1B in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
204 kDa, 199 kDa
Calculated molecular weight
79686 MW
Background of Kif1b
Kinesin-like protein KIF1B is a protein that in humans is encoded by the KIF1B gene. This gene encodes a motor protein that transports mitochondria and synaptic vesicle precursors. Mutations in this gene cause Charcot-Marie-Tooth disease, type 2A1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02951-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, rat brain tissue, mouse skeletal muscle tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KIF1B using anti-KIF1B antibody (A02951-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF1B antigen affinity purified polyclonal antibody (Catalog # A02951-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF1B at approximately 204 kDa or 199 kDa. The expected band size for KIF1B is at 204 kDa.
Protein Target Info & Infographic
Gene/Protein Information For KIF1B (Source: Uniprot.org, NCBI)
Gene Name
KIF1B
Full Name
Kinesin-like protein KIF1B
Weight
79686 MW
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
Caspase-4; CASP-4 Kif1b|A530096N05Rik, AI448212, AI506502, D4Mil, D4Mil1e, KIF1Bp1, KIF1Bp130, KIF1Bp2, KIF1Bp204|kinesin family member 1B|kinesin-like protein KIF1B|N-3 kinesin|kinesin heavy chain member 1B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KIF1B, check out the KIF1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KIF1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KIF1B Antibody Picoband® (A02951-1)
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