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Product Info Summary
| SKU: | PB9653 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Keratocan Antibody Picoband®
SKU/Catalog Number
PB9653
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Keratocan Antibody Picoband® catalog # PB9653. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Keratocan Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9653)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Keratocan, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9653 is reactive to KERA in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
40509 MW
Background of KERA
Keratocan (KTN), also known as keratan sulfate proteoglycan keratocan, is a protein that in humans is encoded by the KERA gene. It is mapped to 12q22. The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2). Keratan sulfate proteoglycans (KSPGs) are members of the small leucine-rich proteoglycan (SLRP) family. KSPGs, particularly keratocan, lumican and mimecan, are important to the transparency of the cornea.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9653 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: Mouse Testis Whole Cell, Mouse Skeletal Muscle Whole Cell, MCF-7 Whole Cell, A549 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Keratocan using anti-Keratocan antibody (PB9653).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Mouse Testis Whole Cell Lysate at 40ug,
Lane 2: Mouse Skeletal Muscle Whole Cell Lysate at 40ug,
Lane 3: MCF-7 Whole Cell Lysate at 40ug,
Lane 4: A549 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Keratocan antigen affinity purified polyclonal antibody (Catalog # PB9653) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Keratocan at approximately 50 kDa. The expected band size for Keratocan is at 40 kDa.
Protein Target Info & Infographic
Gene/Protein Information For KERA (Source: Uniprot.org, NCBI)
Gene Name
KERA
Full Name
Keratocan
Weight
40509 MW
Superfamily
small leucine-rich proteoglycan (SLRP) family
Alternative Names
Keratocan;KTN;Keratan sulfate proteoglycan keratocan;KERA;SLRR2B; KERA CNA2, KTN, SLRR2B keratocan keratocan|keratan sulfate proteoglycan keratocan
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KERA, check out the KERA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KERA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Keratocan Antibody Picoband® (PB9653)
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Customer Q&As
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6 Customer Q&As for Anti-Keratocan Antibody Picoband®
Question
My question regarding product PB9653, anti-Keratocan antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9653 anti-Keratocan antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-03
Question
Does anti-Keratocan antibody PB9653 work on monkey WB with duodenum?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
Our lab technicians have not validated anti-Keratocan antibody PB9653 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Keratocan antibody PB9653 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey duodenum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-Keratocan antibody PB9653. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-05
Question
Is there a BSA free version of anti-Keratocan antibody PB9653 available?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Keratocan antibody PB9653 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-25
Question
We are currently using anti-Keratocan antibody PB9653 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
The anti-Keratocan antibody (PB9653) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-01
Question
I see that the anti-Keratocan antibody PB9653 works with WB, what is the protocol used to produce the result images on the product page?
R. Baker
Verified customer
Asked: 2015-08-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-08-21
