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Product Info Summary
| SKU: | A11990-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-KappaB ras1 NKIRAS1 Antibody
SKU/Catalog Number
A11990-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-KappaB ras1 NKIRAS1 Antibody (Catalog # A11990-1). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse.
Storage & Handling
KappaB ras1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-KappaB ras1 NKIRAS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11990-1)
Host
Rabbit
Contents
KappaB ras1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
KappaB ras1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human KappaB ras1. The immunogen is located within the last 50 amino acids of KappaB ras1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody is predicted to have no cross-reactivity to other Slitrk proteins.
Reactive Species
A11990-1 is reactive to NKIRAS1 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
21643 MW
Background of NKIRAS1
KappaB ras-1 (κB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IκB (IκB), an inhibitor of the transcription factor NF-κB. IκB exists in two homologous forms, IκB-alpha and IκB-beta, although IκB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IκB-alpha-NF-κB complexes can shuttle in and out of the nucleus, IκB-beta-NF-κB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IκB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IκB-beta and regulating the rate of degradation of IκB-beta. This antibody is specific for kappaB-ras1 and has no cross-reactivity to kappaB-ras2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11990-1 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
KappaB ras1 antibody can be used for detection of KappaB ras1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of KappaB ras1 in RAW264.7 cell lysate with KappaB ras1 antibody at (A) 0.5
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Immunocytochemistry of KappaB-ras1 in RAW264.7 cells with KappaB-ras1 antibody at 1 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For NKIRAS1 (Source: Uniprot.org, NCBI)
Gene Name
NKIRAS1
Full Name
NF-kappa-B inhibitor-interacting Ras-like protein 1
Weight
21643 MW
Superfamily
small GTPase superfamily
Alternative Names
KBRAS1, kappaB-Ras1, KBRAS1, NF-kappa-B inhibitor-interacting Ras-like protein 1, I-kappa-B-interacting Ras-like protein 1, Kappa B-Ras protein 1 NKIRAS1 KBRAS1, kappaB-Ras1 NFKB inhibitor interacting Ras like 1 NF-kappa-B inhibitor-interacting Ras-like protein 1|I-kappa-B-interacting Ras-like protein 1|NFKB inhibitor interacting Ras-like protein 1|kappa B-Ras protein 1|kappa B-ras 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NKIRAS1, check out the NKIRAS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NKIRAS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KappaB ras1 NKIRAS1 Antibody (A11990-1)
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