Anti-K6PP Antibody

PFKP antibody

Boster Bio Anti-K6PP Antibody (Catalog# A30471). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.

Product Info Summary

SKU: A30471
Size: 100ul
Reactive Species: Human, Monkey, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, ICC, WB

Product Name

Anti-K6PP Antibody

View all PFKP Antibodies

SKU/Catalog Number

A30471

Size

100ul

Form

Liquid

Description

Boster Bio Anti-K6PP Antibody (Catalog# A30471). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-K6PP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30471)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human K6PP. Immunogen sequence location: 341-390

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30471 is reactive to PFKP in Human, Monkey, Mouse, Rat

Reconstitution

Observed Molecular Weight

72 kDa

Calculated molecular weight

85596 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A30471 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For PFKP (Source: Uniprot.org, NCBI)

Gene Name

PFKP

Full Name

ATP-dependent 6-phosphofructokinase, platelet type

Weight

85596 MW

Superfamily

phosphofructokinase type A (PFKA) family

Alternative Names

PFKP;PFKF;6-phosphofructokinase type C;6-phosphofructokinase; platelet type;Phosphofructo-1-kinase isozyme C;PFK-C;Phosphofructokinase 1;Phosphohexokinase PFKP ATP-PFK, PFK-C, PFK-P, PFKF phosphofructokinase, platelet ATP-dependent 6-phosphofructokinase, platelet type|6-phosphofructokinase type C|6-phosphofructokinase, platelet type|phosphofructo-1-kinase isozyme C|phosphofructokinase 1|phosphohexokinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PFKP, check out the PFKP Infographic

PFKP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PFKP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-K6PP Antibody

Question

Does A30471 anti-K6PP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Taylor

Verified customer

Asked: 2018-10-23

Answer

You can see on the product datasheet, A30471 anti-K6PP antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-10-23

Question

Can you help my question with product A30471, anti-K6PP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-06-05

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30471 anti-K6PP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-06-05

Question

We are currently using anti-K6PP antibody A30471 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2017-10-06

Answer

The anti-K6PP antibody (A30471) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-06

Order DetailsPrice
A30471

100 μL

$335

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A30471
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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