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Product Info Summary
| SKU: | A06353-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-K Cadherin/CDH6 Antibody Picoband®
View all Cadherin-6/KCAD Antibodies
SKU/Catalog Number
A06353-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-K Cadherin/CDH6 Antibody Picoband® catalog # A06353-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-K Cadherin/CDH6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06353-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human K Cadherin/CDH6 recombinant protein (Position: N153-H424).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06353-1 is reactive to CDH6 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
88 kDa
Calculated molecular weight
26056 MW
Background of Cadherin-6/KCAD
Cadherin-6 is a protein that in humans is encoded by the CDH6 gene. This gene encodes a member of the cadherin superfamily. Cadherins are membrane glycoproteins that mediate homophilic cell-cell adhesion and play critical roles in cell differentiation and morphogenesis. The encoded protein is a type II cadherin and may play a role in kidney development as well as endometrium and placenta formation. Decreased expression of this gene may be associated with tumor growth and metastasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06353-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell, human A549 whole cell, human U20S whole cell, human U-87MG whole cell, human A431 whole cell, rat lung tissue, mouse lung tissue
IHC: human ovarian cancer tissue
FCM: PC-3 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of K Cadherin/CDH6 using anti-K Cadherin/CDH6 antibody (A06353-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human U-87MG whole cell lysates,
Lane 5: human A431 whole cell lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-K Cadherin/CDH6 antigen affinity purified polyclonal antibody (Catalog # A06353-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for K Cadherin/CDH6 at approximately 88KD. The expected band size for K Cadherin/CDH6 is at 88KD.
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Figure 2. IHC analysis of K Cadherin/CDH6 using anti-K Cadherin/CDH6 antibody (A06353-1).
K Cadherin/CDH6 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-K Cadherin/CDH6 Antibody (A06353-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Flow Cytometry analysis of PC-3 cells using anti-K Cadherin/CDH6 antibody (A06353-1).
Overlay histogram showing PC-3 cells stained with A06353-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-K Cadherin/CDH6 Antibody (A06353-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CDH6 (Source: Uniprot.org, NCBI)
Gene Name
CDH6
Full Name
Cadherin-6
Weight
26056 MW
Alternative Names
Rho-related GTP-binding protein Rho6;Rho family GTPase 1;Rnd1;RND1;RHO6; CDH6 CAD6, KCAD cadherin 6 cadherin-6|cadherin 6, type 2, K-cadherin (fetal kidney)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDH6, check out the CDH6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDH6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-K Cadherin/CDH6 Antibody Picoband® (A06353-1)
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