Product Info Summary
SKU: | A11503-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ITPRIP Antibody Picoband™
SKU/Catalog Number
A11503-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-ITPRIP Antibody Picoband™ catalog # A11503-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ITPRIP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11503-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human ITPRIP recombinant protein (Position: R161-Q542). Human ITPRIP shares 78.1% amino acid (aa) sequence identity with mouse ITPRIP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A11503-1 is reactive to ITPRIP in Human, Mouse, Rat
Applications
A11503-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
80 kDa
Calculated molecular weight
52588 MW
Background of ITPRIP
This gene encodes a membrane-associated protein that binds the inositol 1,4,5-trisphosphate receptor (ITPR). The encoded protein enhances the sensitivity of ITPR to intracellular calcium signaling. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Immunohistochemistry, 2-5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a11503 1 itprip primary antibodies wb testing 1 a11503 1 itprip primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a11503-1-itprip-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of ITPRIP using anti-ITPRIP antibody (A11503-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissues lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPRIP antigen affinity purified polyclonal antibody (Catalog # A11503-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITPRIP at approximately 80 kDa. The expected band size for ITPRIP is at 62 kDa.
![a11503 1 itprip primary antibodies ihc testing 2 a11503 1 itprip primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a11503-1-itprip-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of ITPRIP using anti-ITPRIP antibody (A11503-1).
ITPRIP was detected in a paraffin-embedded section of human pancreas ductal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITPRIP Antibody (A11503-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a11503 1 itprip primary antibodies ihc testing 3 a11503 1 itprip primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/1/a11503-1-itprip-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of ITPRIP using anti-ITPRIP antibody (A11503-1).
ITPRIP was detected in a paraffin-embedded section of human pancreas ductal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITPRIP Antibody (A11503-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a11503 1 itprip primary antibodies if testing 4 a11503 1 itprip primary antibodies if testing 4](https://www.bosterbio.com/media/catalog/product/a/1/a11503-1-itprip-primary-antibodies-if-testing-4.jpg)
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Figure 4. IF analysis of ITPRIP using anti-ITPRIP antibody (A11503-1).
ITPRIP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ITPRIP Antibody (A11503-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For ITPRIP (Source: Uniprot.org, NCBI)
Gene Name
ITPRIP
Full Name
Inositol 1,4,5-trisphosphate receptor-interacting protein
Weight
52588 MW
Superfamily
ITPRIP family
Alternative Names
bA127L20.2; DANGERKIAA1754bA127L20; inositol 14,5-triphosphate receptor interacting protein; inositol 14,5-triphosphate receptor-interacting protein; Protein DANGER ITPRIP DANGER, KIAA1754, bA127L20, bA127L20.2 inositol 1,4,5-trisphosphate receptor interacting protein inositol 1,4,5-trisphosphate receptor-interacting protein|inositol 1,4,5-triphosphate receptor-interacting protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITPRIP, check out the ITPRIP Infographic
![ITPRIP infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITPRIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ITPRIP Antibody Picoband™ (A11503-1)
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