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Product Info Summary
| SKU: | A03614 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-IRGM Antibody
SKU/Catalog Number
A03614
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IRGM Antibody (Catalog # A03614). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
IRGM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IRGM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03614)
Host
Rabbit
Contents
IRGM Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IRGM antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human IRGM. The immunogen is located within amino acids 30 - 80 of IRGM.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
IL-37 antibody is human specific. Multiple isoforms of IL-37 are known to exist; this antibody will only detect the largest isoform. IL-37 antibody is predicted to not cross-react with IL-36.
Reactive Species
A03614 is reactive to IRGM in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
20142 MW
Background of Irgm
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn's disease. IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. In mouse, IRGM belongs to a family of gamma-interferon-induced GTP-binding proteins of approximately 48 kDa that also includes IRGM2 and IRGM3; this antibody may also recognize these proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03614 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IRGM antibody can be used for detection of IRGM by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IRGM expression in SK-N-SH cell lysate with IRGM antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of CD4 in human thymus tissue with CD4 antibody at 5 μg/ml.
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Immunofluorescence of IRGM in Human Brain tissue with IRGM antibody at 20 μg/mL.
Green: IRGM Antibody (A03614)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For IRGM (Source: Uniprot.org, NCBI)
Gene Name
IRGM
Full Name
Immunity-related GTPase family M protein
Weight
20142 MW
Superfamily
TRAFAC class dynamin-like GTPase superfamily
Alternative Names
IFI1, IRGM1, LRG47, LRG-47, IFI1, Immunity-related GTPase family M protein, Immunity-related GTPase family M protein 1 Irgm|Ifggd3, Ifi1|immunity-related GTPase M|immunity-related GTPase family M protein|immunity-related GTPase family, M|interferon inducible protein 1|interferon-gamma-inducible GTPase Ifggd3 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRGM, check out the IRGM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRGM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRGM Antibody (A03614)
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