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Product Info Summary
| SKU: | A08098 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-IPT/TRIT1 Antibody Picoband®
SKU/Catalog Number
A08098
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IPT/TRIT1 Antibody Picoband® catalog # A08098. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-IPT/TRIT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08098)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IPT/TRIT1 recombinant protein (Position: L16-K415).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08098 is reactive to TRIT1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
53 kDa
Calculated molecular weight
17136 MW
Background of TRIT1
tRNA isopentenyltransferase, mitochondrial is an enzyme that in humans is encoded by the TRIT1 gene. This gene encodes a protein that that is targeted to the mitochondrion and modifies transfer RNAs (tRNAs) by adding a dimethylallyl group onto the adenine at position 37. This modification is important for maintaining the correct reading frame during protein translation. This gene is considered a tumor suppressor and its expression can decrease cell growth. Alternative splicing results in multiple transcripts variants, most of which are likely non-functional.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08098 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, human Raji whole cell, human K562 whole cell, human RT4 whole cell, human HEL whole cell, human Hela whole cell
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IPT/TRIT1 using anti-IPT/TRIT1 antibody (A08098).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: human HEL whole cell lysates,
Lane 6: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IPT/TRIT1 antigen affinity purified polyclonal antibody (Catalog # A08098) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IPT/TRIT1 at approximately 53 kDa. The expected band size for IPT/TRIT1 is at 53 kDa.
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Figure 2. Flow Cytometry analysis of 293T cells using anti-IPT/TRIT1 antibody (A08098).
Overlay histogram showing 293T cells stained with A08098 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IPT/TRIT1 Antibody (A08098, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TRIT1 (Source: Uniprot.org, NCBI)
Gene Name
TRIT1
Full Name
tRNA dimethylallyltransferase
Weight
17136 MW
Superfamily
IPP transferase family
Alternative Names
Mediator of RNA polymerase II transcription subunit 8; Activator-recruited cofactor 32 kDa component; ARC32; Mediator complex subunit 8; MED8; TRIT1 COXPD35, GRO1, IPPT, IPT, IPTase, MOD5, hGRO1 tRNA isopentenyltransferase 1 tRNA dimethylallyltransferase|IPP transferase|isopentenyl-diphosphate:tRNA isopentenyltransferase|tRNA dimethylallyltransferase, mitochondrial|tRNA isopentenylpyrophosphate transferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIT1, check out the TRIT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IPT/TRIT1 Antibody Picoband® (A08098)
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