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Product Info Summary
| SKU: | A30465 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC |
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Product info
Product Name
Anti-IPKA PKIA Antibody
SKU/Catalog Number
A30465
Size
100ul
Form
Liquid
Description
Boster Bio Anti-IPKA PKIA Antibody (Catalog# A30465). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IPKA PKIA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30465)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human IPKA. Immunogen sequence location: 10-59
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30465 is reactive to PKIA in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
7989 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30465 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using IPKA Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of HeLa cells, using IPKA Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For PKIA (Source: Uniprot.org, NCBI)
Gene Name
PKIA
Full Name
cAMP-dependent protein kinase inhibitor alpha
Weight
7989 MW
Superfamily
PKI family
Alternative Names
PKIA;PRKACN1;cAMP-dependent protein kinase inhibitor alpha;PKI-alpha;cAMP-dependent protein kinase inhibitor; muscle/brain isoform PKIA PRKACN1 cAMP-dependent protein kinase inhibitor alpha cAMP-dependent protein kinase inhibitor alpha|PKI-alpha|protein kinase (cAMP-dependent, catalytic) inhibitor alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PKIA, check out the PKIA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PKIA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IPKA PKIA Antibody (A30465)
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Customer Q&As
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4 Customer Q&As for Anti-IPKA PKIA Antibody
Question
I was wanting to use your anti-IPKA antibody for IHC for human biceps brachii on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human biceps brachii identification?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
You can see on the product datasheet, A30465 anti-IPKA antibody has been tested for IF, IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human biceps brachii in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-16
Question
We are currently using anti-IPKA antibody A30465 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-18
Answer
The anti-IPKA antibody (A30465) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-18
Question
Is a blocking peptide available for product anti-IPKA antibody (A30465)?
C. Li
Verified customer
Asked: 2015-05-05
Answer
We do provide the blocking peptide for product anti-IPKA antibody (A30465). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-05-05
Question
Would anti-IPKA antibody A30465 work for IHC with biceps brachii?
O. Evans
Verified customer
Asked: 2013-09-02
Answer
According to the expression profile of biceps brachii, PKIA is highly expressed in biceps brachii. So, it is likely that anti-IPKA antibody A30465 will work for IHC with biceps brachii.
Boster Scientific Support
Answered: 2013-09-02
