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Product Info Summary
| SKU: | M00393-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Mouse |
| Application: | ELISA |
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Product info
Product Name
Anti-Interferon gamma IFNG Antibody (monoclonal, 8E9)
SKU/Catalog Number
M00393-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interferon gamma IFNG Antibody (monoclonal, 8E9) catalog # M00393-2. Tested in ELISA applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interferon gamma IFNG Antibody (monoclonal, 8E9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00393-2)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
Clone: 8E9
Isotype
Mouse IgG1
Immunogen
E. coli-derived rat Interferon gamma recombinant protein (Position: Q23-C156). Rat Interferon gamma shares 38% and 86% amino acid (aa) sequence identity with human and mouse Interferon gamma, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00393-2 is reactive to IFNG in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
53 kDa
Calculated molecular weight
19.348kDa
Background of IFN-gamma
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00393-2 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA (Cap), 1-5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Sandwich ELISA - Recombinant rat Interferon gamma protein standard curve.
Use in combination with reagents from Rat Interferon gamma ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0374).
Protein Target Info & Infographic
Gene/Protein Information For IFNG (Source: Uniprot.org, NCBI)
Gene Name
IFNG
Full Name
Interferon gamma
Weight
19.348kDa
Superfamily
type II (or gamma) interferon family
Alternative Names
Interferon gamma; IFN-gamma; IFNG IFNG IFG, IFI, IMD69 interferon gamma interferon gamma|IFN-gamma|immune interferon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IFNG, check out the IFNG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IFNG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interferon gamma IFNG Antibody (monoclonal, 8E9) (M00393-2)
Hello CJ!
M00393-2 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Somatostatin Improved B Cells Mature in Macaques during Intestinal Ischemia-Reperfusion
Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques
Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Interferon gamma IFNG Antibody (monoclonal, 8E9)
Question
I was wanting to use your anti-Interferon gamma antibody (monoclonal, 8E9) for ELISA for rat blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat blood identification?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
As indicated on the product datasheet, M00393-2 anti-Interferon gamma antibody (monoclonal, 8E9) has been validated for ELISA on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-30
Question
My lab would like to test anti-Interferon gamma antibody (monoclonal, 8E9) M00393-2 on rat blood for research purposes, then I may be interested in using anti-Interferon gamma antibody (monoclonal, 8E9) M00393-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The products we sell, including anti-Interferon gamma antibody (monoclonal, 8E9) M00393-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-18
Question
We are currently using anti-Interferon gamma antibody (monoclonal, 8E9) M00393-2 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
The anti-Interferon gamma antibody (monoclonal, 8E9) (M00393-2) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-07
Question
We have been able to see staining in rat leukocyte. Any tips? Is anti-Interferon gamma antibody (monoclonal, 8E9) supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
According to literature leukocyte does express IFNG. According to Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-21
Question
Is this M00393-2 anti-Interferon gamma antibody (monoclonal, 8E9) reactive to the isotypes of IFNG?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
The immunogen of M00393-2 anti-Interferon gamma antibody (monoclonal, 8E9) is E. coli-derived rat Interferon gamma recombinant protein (Position: Q23-C156). Rat Interferon gamma shares 38% and 86% amino acid (aa) sequence identity with human and mouse Interferon gamma, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-26
Question
Is a blocking peptide available for product anti-Interferon gamma antibody (monoclonal, 8E9) (M00393-2)?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
We do provide the blocking peptide for product anti-Interferon gamma antibody (monoclonal, 8E9) (M00393-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-17
Question
My question regarding product M00393-2, anti-Interferon gamma antibody (monoclonal, 8E9). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00393-2 anti-Interferon gamma antibody (monoclonal, 8E9), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-17
Question
I see that the anti-Interferon gamma antibody (monoclonal, 8E9) M00393-2 works with ELISA, what is the protocol used to produce the result images on the product page?
O. Parker
Verified customer
Asked: 2018-06-26
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-26
Question
Do you have a BSA free version of anti-Interferon gamma antibody (monoclonal, 8E9) M00393-2 available?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Interferon gamma antibody (monoclonal, 8E9) M00393-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-24
Question
My colleagues were content with the WB result of your anti-Interferon gamma antibody (monoclonal, 8E9). However we have observed positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?
A. Lewis
Verified customer
Asked: 2018-04-05
Answer
According to literature, blood does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-04-05
Question
Does anti-Interferon gamma antibody (monoclonal, 8E9) M00393-2 work for ELISA with blood?
P. Johnson
Verified customer
Asked: 2016-02-23
Answer
According to the expression profile of blood, IFNG is highly expressed in blood. So, it is likely that anti-Interferon gamma antibody (monoclonal, 8E9) M00393-2 will work for ELISA with blood.
Boster Scientific Support
Answered: 2016-02-23
Question
Does M00393-2 anti-Interferon gamma antibody (monoclonal, 8E9) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Lewis
Verified customer
Asked: 2015-11-09
Answer
You can see on the product datasheet, M00393-2 anti-Interferon gamma antibody (monoclonal, 8E9) as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-11-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Interferon gamma antibody (monoclonal, 8E9) M00393-2. Let me know if you need anything else.
K. Miller
Verified customer
Asked: 2014-10-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-17
Question
Here is the WB image, lot number and protocol we used for blood using anti-Interferon gamma antibody (monoclonal, 8E9) M00393-2. Please let me know if you require anything else.
C. Jones
Verified customer
Asked: 2014-04-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-17
