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Product Info Summary
| SKU: | A04954-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-INPP5F Antibody Picoband®
SKU/Catalog Number
A04954-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-INPP5F Antibody Picoband® catalog # A04954-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-INPP5F Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04954-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human INPP5F recombinant protein (Position: H317-Q1112). Human INPP5F shares 87.6% amino acid (aa) sequence identity with mouse INPP5F.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04954-1 is reactive to INPP5F in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
128 kDa
Calculated molecular weight
128.407kDa
Background of INPP5F
Inositol polyphosphate-5-phosphatase F is a protein that in humans is encoded by the INPP5F gene. The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase and contains a Sac domain. The activity of this protein is specific for phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04954-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human SH-SY5Y whole cell, human SIHA whole cell, human U251 whole cell, human Caco-2 whole cell
FCM: Daudi cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of INPP5F using anti-INPP5F antibody (A04954-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human SIHA whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP5F antigen affinity purified polyclonal antibody (Catalog # A04954-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPP5F at approximately 128 kDa. The expected band size for INPP5F is at 128 kDa.
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Figure 2. Flow Cytometry analysis of Daudi cells using anti-INPP5F antibody (A04954-1).
Overlay histogram showing Daudi cells stained with A04954-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-INPP5F Antibody (A04954-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For INPP5F (Source: Uniprot.org, NCBI)
Gene Name
INPP5F
Full Name
Phosphatidylinositide phosphatase SAC2
Weight
128.407kDa
Alternative Names
EC 3.1.3; EC 3.1.3.-; EC 3.1.3.16; EC 3.1.3.36; FLJ13081; hSAC2; Inositol polyphosphate 5-phosphatase F; inositol polyphosphate-5-phosphatase F; KIAA0966MGC59773; MGC131851; phosphatidylinositide phosphatase SAC2; Sac domain-containing inositol phosphatase 2; Sac domain-containing phosphoinositide 5-phosphatase 2; SAC2MSTPO47 INPP5F MSTP007, MSTPO47, SAC2, hSAC2 inositol polyphosphate-5-phosphatase F phosphatidylinositide phosphatase SAC2|Sac domain-containing inositol phosphatase 2|sac domain-containing phosphoinositide 4-phosphatase 2|sac domain-containing phosphoinositide 5-phosphatase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INPP5F, check out the INPP5F Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INPP5F: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-INPP5F Antibody Picoband® (A04954-1)
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