Product Info Summary
SKU: | A11475-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-INO80B Antibody Picoband®
SKU/Catalog Number
A11475-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-INO80B Antibody Picoband® catalog # A11475-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-INO80B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11475-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human INO80B recombinant protein (Position: Q61-Q340). Human INO80B shares 94.3% amino acid (aa) sequence identity with mouse INO80B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A11475-1 is reactive to INO80B in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
52588 MW
Background of Ino80b
INO80 complex subunit B is a protein that in humans is encoded by the INO80B gene. This gene encodes a subunit of an ATP-dependent chromatin remodeling complex, INO80, which plays a role in DNA and nucleosome-activated ATPase activity and ATP-dependent nucleosome sliding. Readthrough transcription of this gene into the neighboring downstream gene, which encodes WW domain-binding protein 1, generates a non-coding transcript.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11475-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human MCF-7 whole cell, human 293T whole cell
FCM: 293T cell, JK cell
Validation Images & Assay Conditions
![a11475 1 ino80b primary antibodies wb testing 1 a11475 1 ino80b primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a11475-1-ino80b-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of INO80B using anti-INO80B antibody (A11475-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INO80B antigen affinity purified polyclonal antibody (Catalog # A11475-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INO80B at approximately 48 kDa. The expected band size for INO80B is at 39,50 kDa.
![a11475 1 ino80b primary antibodies fcm testing 2 a11475 1 ino80b primary antibodies fcm testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a11475-1-ino80b-primary-antibodies-fcm-testing-2.png)
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Figure 2. Flow Cytometry analysis of 293T cells using anti-INO80B antibody (A11475-1).
Overlay histogram showing 293T cells stained with A11475-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-INO80B Antibody (A11475-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
![a11475 1 ino80b primary antibodies fcm testing 3 a11475 1 ino80b primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/1/a11475-1-ino80b-primary-antibodies-fcm-testing-3.png)
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Figure 3. Flow Cytometry analysis of JK cells using anti-INO80B antibody (A11475-1).
Overlay histogram showing JK cells stained with A11475-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-INO80B Antibody (A11475-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For INO80B (Source: Uniprot.org, NCBI)
Gene Name
INO80B
Full Name
INO80 complex subunit B
Weight
52588 MW
Alternative Names
70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INO80B, check out the INO80B Infographic
![INO80B infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INO80B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-INO80B Antibody Picoband® (A11475-1)
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