Anti-Importin subunit alpha-7 KPNA6 Antibody

Product Name

Anti-Importin subunit alpha-7 KPNA6 Antibody

View all Kpna6 Antibodies

SKU/Catalog Number

A07295

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Importin subunit alpha-7 KPNA6 Antibody (Catalog # A07295). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

KPNA6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Importin subunit alpha-7 KPNA6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07295)

Host

Rabbit

Contents

KPNA6 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

KPNA6 antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of human KPNA6. The immunogen is located within amino acids 70 - 120 of KPNA6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of BICD1 are known to exist; this antibody will only recognize the larger isoform.

Reactive Species

A07295 is reactive to KPNA6 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

60030 MW

Background of Kpna6

Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA6 belongs to a subfamily within the KPNA family that also includes IPOA5&6. Down-regulation of KPNA6 by RNAi expression in HeLa cells strongly inhibited cell proliferation, possibly due to blocking the nuclear import of specific factors essential for cell growth and proliferation. KPNA6 also interacts with the Rev protein of HIV-1 and promote its nuclear import.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07295 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

KPNA6 antibody can be used for detection of KPNA6 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For KPNA6 (Source: Uniprot.org, NCBI)

Alternative Names

IPOA7, KPNA7, IPOA7, Importin subunit alpha-7, Karyopherin subunit alpha-6 Kpna6|I, IPOA7, Kpna5, NP, NPI-2|karyopherin (importin) alpha 6|importin subunit alpha-7|importin alpha 7|importin alpha-S2|karyopherin (importin) alpha 5|karyopherin subunit alpha-6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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