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Product Info Summary
| SKU: | A00918 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-IL-12A Antibody
SKU/Catalog Number
A00918
Size
100μl
Form
Liquid
Description
Boster Bio Anti-IL-12A Antibody catalog # A00918. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-12A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00918)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human IL-12A. AA range:16-65
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00918 is reactive to IL12A in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
24874 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00918 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC-p 1:50-300
ELISA 1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HeLa cells using IL-12A Polyclonal antibody.
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Western blot analysis of lysate from HeLa cells, using IL-12A antibody.
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Immunohistochemistry analysis of IL-12A antibody in paraffin-embedded human brain tissue.
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Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Protein Target Info & Infographic
Gene/Protein Information For IL12A (Source: Uniprot.org, NCBI)
Gene Name
IL12A
Full Name
Interleukin-12 subunit alpha
Weight
24874 MW
Superfamily
IL-6 superfamily
Alternative Names
Interleukin-12 subunit alpha;IL-12A;Cytotoxic lymphocyte maturation factor 35 kDa subunit;CLMF p35;IL-12 subunit p35;NK cell stimulatory factor chain 1;NKSF1;IL12A;NKSF1; IL12A CLMF, IL-12A, NFSK, NKSF1, P35 interleukin 12A interleukin-12 subunit alpha|CLMF p35|IL-12, subunit p35|IL35 subunit|NF cell stimulatory factor chain 1|NK cell stimulatory factor chain 1|cytotoxic lymphocyte maturation factor 1, p35|cytotoxic lymphocyte maturation factor 35 kDa subunit|interleukin 12, p35|interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)|interleukin-12 alpha chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL12A, check out the IL12A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL12A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-12A Antibody (A00918)
Hello CJ!
A00918 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiang Peng-Fei,Peng Jun,Tan Han-Yu,Ou-Yang Yun,Qin Gen-Yan,Peng Qing-Hua,Effects of Buddlejae Flos Granules on Inflammatory Factors TGF-β1, NF-κB, IL-10 and IL-12 in Lacrimal Gland Cells of Castrated Male Rabbits,Digital Chinese Medicine,Volume 2,Issue 2,2019,Pages 97-104,ISSN 2589-3777,https://doi.org/10.1016/j.dcmed.2019.09.004.
Species: New Zealand White Rabbit
A00918 usage in article: APP:IHC, SAMPLE:LACRIMAL GLAND CELL, DILUTION:NA
Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-IL-12A Antibody
Question
Is there a BSA free version of anti-IL-12A antibody A00918 available?
W. Kulkarni
Verified customer
Asked: 2019-12-19
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-12A antibody A00918 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-19
Question
I see that the anti-IL-12A antibody A00918 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-30
Question
I was wanting to use your anti-IL-12A antibody for WB for rat lower esophagus mucosa on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat lower esophagus mucosa identification?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
You can see on the product datasheet, A00918 anti-IL-12A antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lower esophagus mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-09
Question
Will anti-IL-12A antibody A00918 work on primate WB with lower esophagus mucosa?
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
Our lab technicians have not tested anti-IL-12A antibody A00918 on primate. You can run a BLAST between primate and the immunogen sequence of anti-IL-12A antibody A00918 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate lower esophagus mucosa in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-29
Question
Is a blocking peptide available for product anti-IL-12A antibody (A00918)?
Verified Customer
Verified customer
Asked: 2018-03-12
Answer
We do provide the blocking peptide for product anti-IL-12A antibody (A00918). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-12
Question
Is this A00918 anti-IL-12A antibody reactive to the isotypes of IL12A?
G. Patel
Verified customer
Asked: 2018-01-02
Answer
The immunogen of A00918 anti-IL-12A antibody is Synthesized peptide derived from the Internal region of human IL-12A. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-02
Question
My question regards to test anti-IL-12A antibody A00918 on rat lower esophagus mucosa for research purposes, then I may be interested in using anti-IL-12A antibody A00918 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Collins
Verified customer
Asked: 2014-11-04
Answer
The products we sell, including anti-IL-12A antibody A00918, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-11-04
