Anti-IKAP ELP1 Antibody

Product Name

Anti-IKAP ELP1 Antibody

View all IKBKAP Antibodies

SKU/Catalog Number

A31687

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-IKAP ELP1 Antibody (Catalog # A31687). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

IKAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-IKAP ELP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31687)

Host

Rabbit

Contents

IKAP Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

IKAP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human IKAP. The immunogen is located within the last 50 amino acids of IKAP.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of IKAP are known two exist, this antibody will detect both isoforms.

Reactive Species

A31687 is reactive to ELP1 in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

150254 MW

Background of IKBKAP

IKAP was initially identified as a scaffold protein of the IκB kinase complex that could bind to IKKα, IKKβ, NF-κB, and the NF-κB-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A31687 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IKAP antibody can be used for detection of IKAP by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Positive Control

Validation Images & Assay Conditions

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Western blot analysis of IKAP in A-20 cell lysate with IKAP antibody at in (A) 0.5

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Immunocytochemistry of IKAP in A-20 cells with IKAP antibody at 1 μg/mL.

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Immunofluorescence of IKAP in A20 cells with IKAP antibody at 20 μg/mL.

Gene/Protein Information For ELP1 (Source: Uniprot.org, NCBI)

Alternative Names

DYS; elongator complex protein 1; ELP1DKFZp781H1425; FD; FLJ12497; IKAPdysautonomia (Riley-Day syndrome, hereditary sensory autonomic neuropathy typeIII); IkappaB kinase complex-associated protein; IKI3; IKK complex-associated protein; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinasecomplex-associated protein; p150; TOT1 ELP1 DYS, FD, IKAP, IKBKAP, IKI3, TOT1 elongator acetyltransferase complex subunit 1 elongator complex protein 1|IKK complex-associated protein|ikappaB kinase complex-associated protein|inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein

For more info on ELP1, check out the ELP1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ELP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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