Anti-Interferon gamma/IFNG Antibody Picoband®

IFN-gamma antibody

Boster Bio Anti-Interferon gamma/IFNG Antibody catalog # PA1493. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 5 publication(s).

Product Info Summary

SKU: PA1493
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Interferon gamma/IFNG Antibody Picoband®

View all IFN-gamma Antibodies

SKU/Catalog Number

PA1493

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interferon gamma/IFNG Antibody catalog # PA1493. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interferon gamma/IFNG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1493)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human IFN gamma.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1493 is reactive to IFNG in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

19 kDa

Calculated molecular weight

19348 MW

Background of IFN-gamma

Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1493 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For IFNG (Source: Uniprot.org, NCBI)

Gene Name

IFNG

Full Name

Interferon gamma

Weight

19348 MW

Superfamily

type II (or gamma) interferon family

Alternative Names

Interferon gamma;IFN-gamma;Immune interferon;IFNG; IFNG IFG, IFI, IMD69 interferon gamma interferon gamma|IFN-gamma|immune interferon

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IFNG, check out the IFNG Infographic

IFNG infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IFNG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1493 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface

Protective effect of carnosine after chronic cerebral hypoperfusion possibly through suppressing astrocyte activation

Somatostatin Improved B Cells Mature in Macaques during Intestinal Ischemia-Reperfusion

Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques

Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.

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3 Customer Q&As for Anti-Interferon gamma/IFNG Antibody Picoband®

Question

We are currently using anti-Interferon gamma/IFNG antibody PA1493 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-07-06

Answer

The anti-Interferon gamma/IFNG antibody (PA1493) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-07-06

Question

We have been able to see staining in human blood. What should we do? Is anti-Interferon gamma/IFNG antibody supposed to stain blood positively?

Verified Customer

Verified customer

Asked: 2017-10-13

Answer

From literature blood does express IFNG. From Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-10-13

Question

We were content with the WB result of your anti-Interferon gamma/IFNG antibody. However we have been able to see positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-09-18

Answer

From what I have seen in literature, blood does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-09-18

Order DetailsPrice
PA1493

100μg

$370
PA1493-10ug

10μg sample (liquid)

$99
PA1493-Biotin

100 μg Biotin conjugated

$570
PA1493-Cy3

100 μg Cy3 conjugated

$570
PA1493-Dylight488

100 μg Dylight488 conjugated

$570
PA1493-Dylight550

100 μg Dylight550 conjugated

$570
PA1493-Dylight594

100 μg Dylight594 conjugated

$570
PA1493-FITC

100 μg FITC conjugated

$570
PA1493-HRP

100 μg HRP conjugated

$570
PA1493-APC

100 μg APC conjugated

$670
PA1493-PE

100 μg PE conjugated

$670
PA1493-iFluor647

100 μg iFluor647 conjugated

$670
PA1493-carrier-free

Carrier Free

$370

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PA1493
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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