Product Info Summary
SKU: | PA1493 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Interferon gamma/IFNG Antibody Picoband®
SKU/Catalog Number
PA1493
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interferon gamma/IFNG Antibody catalog # PA1493. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interferon gamma/IFNG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1493)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IFN gamma.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1493 is reactive to IFNG in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
19 kDa
Calculated molecular weight
19348 MW
Background of IFN-gamma
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1493 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-IFN gamma antibody, PA1493, Western blotting
All lanes: Anti IFN gamma (PA1493) at 0.5ug/ml
Lane 1: Recombinant Human IFN gamma Protein 10ng
Lane 2: Recombinant Human IFN gamma Protein 5ng
Lane 3: Recombinant Human IFN gamma Protein 2.5ng
Lane 4: Recombinant Human IFN gamma Protein 1.25ng
Predicted bind size: 19KD
Observed bind size: 19KD
Protein Target Info & Infographic
Gene/Protein Information For IFNG (Source: Uniprot.org, NCBI)
Gene Name
IFNG
Full Name
Interferon gamma
Weight
19348 MW
Superfamily
type II (or gamma) interferon family
Alternative Names
Interferon gamma;IFN-gamma;Immune interferon;IFNG; IFNG IFG, IFI, IMD69 interferon gamma interferon gamma|IFN-gamma|immune interferon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IFNG, check out the IFNG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IFNG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interferon gamma/IFNG Antibody Picoband® (PA1493)
Hello CJ!
PA1493 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface
Protective effect of carnosine after chronic cerebral hypoperfusion possibly through suppressing astrocyte activation
Somatostatin Improved B Cells Mature in Macaques during Intestinal Ischemia-Reperfusion
Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques
Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Interferon gamma/IFNG Antibody Picoband®
Question
We are currently using anti-Interferon gamma/IFNG antibody PA1493 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
The anti-Interferon gamma/IFNG antibody (PA1493) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-06
Question
We have been able to see staining in human blood. What should we do? Is anti-Interferon gamma/IFNG antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2017-10-13
Answer
From literature blood does express IFNG. From Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-10-13
Question
We were content with the WB result of your anti-Interferon gamma/IFNG antibody. However we have been able to see positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-09-18
Answer
From what I have seen in literature, blood does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-09-18