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Product Info Summary
| SKU: | M00510 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-IDH2 Monoclonal Antibody
SKU/Catalog Number
M00510
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IDH2 Monoclonal Antibody catalog # M00510. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IDH2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00510)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACFO-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IDH2 Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00510 is reactive to IDH2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
30 kDa
Calculated molecular weight
85659 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00510 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:100
Positive Control
WB: human MOLT-4 whole cell, human Jurkat whole cell, human 293T whole cell, human K562 whole cell, rat kidney tissue, rat liver tissue, mouse kidney tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IDH2 using anti-IDH2 antibody (M00510).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse liver tissue lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH2 antigen affinity purified monoclonal antibody (Catalog # M00510) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDH2 at approximately 45 kDa. The expected band size for IDH2 is at 51 kDa.
Click image to see more details
Figure 2. IHC analysis of IDH2 using anti-IDH2 antibody (M00510).
IDH2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IDH2 Antibody (M00510) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of IDH2 using anti-IDH2 antibody (M00510).
IDH2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IDH2 Antibody (M00510) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For IDH2 (Source: Uniprot.org, NCBI)
Gene Name
IDH2
Full Name
Isocitrate dehydrogenase [NADP], mitochondrial
Weight
85659 MW
Superfamily
isocitrate and isopropylmalate dehydrogenases family
Alternative Names
Glucocorticoid receptor;GR;Nuclear receptor subfamily 3 group C member 1;NR3C1;GRL; IDH2 D2HGA2, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-IDH isocitrate dehydrogenase (NADP(+)) 2 isocitrate dehydrogenase [NADP], mitochondrial|NADP(+)-specific ICDH|isocitrate dehydrogenase (NADP(+)) 2, mitochondrial|isocitrate dehydrogenase 2 (NADP+), mitochondrial|oxalosuccinate decarboxylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IDH2, check out the IDH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IDH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IDH2 Monoclonal Antibody (M00510)
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No publications found for M00510
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Customer Q&As
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14 Customer Q&As for Anti-IDH2 Monoclonal Antibody
Question
Will anti-IDH2 Monoclonal antibody M00510 work for WB with colon skin?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
According to the expression profile of colon skin, IDH2 is highly expressed in colon skin. So, it is likely that anti-IDH2 Monoclonal antibody M00510 will work for WB with colon skin.
Boster Scientific Support
Answered: 2020-04-24
Question
My question regarding product M00510, anti-IDH2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00510 anti-IDH2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-22
Question
Would M00510 anti-IDH2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
It shows on the product datasheet, M00510 anti-IDH2 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-21
Question
Is a blocking peptide available for product anti-IDH2 Monoclonal antibody (M00510)?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
We do provide the blocking peptide for product anti-IDH2 Monoclonal antibody (M00510). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-07
Question
We are currently using anti-IDH2 Monoclonal antibody M00510 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The anti-IDH2 Monoclonal antibody (M00510) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-24
Question
Is there a BSA free version of anti-IDH2 Monoclonal antibody M00510 available?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-IDH2 Monoclonal antibody M00510 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-16
Question
Please see the WB image, lot number and protocol we used for colon skin using anti-IDH2 Monoclonal antibody M00510. Please let me know if you require anything else.
O. Anderson
Verified customer
Asked: 2019-08-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-01
Question
We have been able to see staining in mouse liver. Do you have any suggestions? Is anti-IDH2 Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
From what I have seen in literature liver does express IDH2. From what I have seen in Uniprot.org, IDH2 is expressed in muscle of leg, heart, brain cortex, heart testis, colon skin, liver, among other tissues. Regarding which tissues have IDH2 expression, here are a few articles citing expression in various tissues:
Brain cortex, Heart, and Testis, Pubmed ID: 14702039
Colon, and Skin, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-07-31
Question
I was wanting to use your anti-IDH2 Monoclonal antibody for WB for mouse colon skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse colon skin identification?
Verified Customer
Verified customer
Asked: 2017-12-06
Answer
As indicated on the product datasheet, M00510 anti-IDH2 Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse colon skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon skin using anti-IDH2 Monoclonal antibody M00510. Let me know if you need anything else.
O. Moore
Verified customer
Asked: 2017-09-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-06
Question
I would like to test anti-IDH2 Monoclonal antibody M00510 on mouse colon skin for research purposes, then I may be interested in using anti-IDH2 Monoclonal antibody M00510 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Li
Verified customer
Asked: 2015-11-10
Answer
The products we sell, including anti-IDH2 Monoclonal antibody M00510, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-11-10
Question
Is this M00510 anti-IDH2 Monoclonal antibody reactive to the isotypes of IDH2?
D. Krishna
Verified customer
Asked: 2014-12-10
Answer
The immunogen of M00510 anti-IDH2 Monoclonal antibody is A synthesized peptide derived from human IDH2 Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-12-10
Question
I see that the anti-IDH2 Monoclonal antibody M00510 works with WB, what is the protocol used to produce the result images on the product page?
D. Parker
Verified customer
Asked: 2014-02-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-02-10
Question
My colleagues were content with the WB result of your anti-IDH2 Monoclonal antibody. However we have observed positive staining in heart testis mitochondrion. using this antibody. Is that expected? Could you tell me where is IDH2 supposed to be expressed?
J. Miller
Verified customer
Asked: 2013-10-02
Answer
From literature, heart testis does express IDH2. Generally IDH2 expresses in mitochondrion. Regarding which tissues have IDH2 expression, here are a few articles citing expression in various tissues:
Brain cortex, Heart, and Testis, Pubmed ID: 14702039
Colon, and Skin, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2013-10-02
