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Product Info Summary
| SKU: | A00510 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-IDH2 Antibody
SKU/Catalog Number
A00510
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IDH2 Antibody (Catalog # A00510). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
IDH2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IDH2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00510)
Host
Rabbit
Contents
IDH2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IDH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human IDH2. The immunogen is located within the first 50 amino acids of IDH2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms are known to exist. The immunogen to this antibody is outside of the Integrin alpha4 recombinant protein fragment and thus will not recognize it.
Reactive Species
A00510 is reactive to IDH2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
50909 MW
Background of IDH2
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Two NADP(+)-dependent isocitrate dehydrogenases have been found as homodimer: IDH1 is predominantly cytosolic and peroxisomal and IDH2 is mitochondrial. Both IDH1 and IDH2 play significant roles in the tricarboxylic acid cycle (TCA), and defects in IDH1 as well as IDH2 have been implicated in the development of glioma as well as other malignancies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00510 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IDH2 antibody can be used for detection of IDH2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IDH2 in human heart tissue lysate with IDH2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of IDH2 in mouse heart tissue with IDH2 antibody at 5 μg/mL.
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Immunofluorescence of IDH2 in mouse heart tissue with IDH2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For IDH2 (Source: Uniprot.org, NCBI)
Gene Name
IDH2
Full Name
Isocitrate dehydrogenase [NADP], mitochondrial
Weight
50909 MW
Superfamily
isocitrate and isopropylmalate dehydrogenases family
Alternative Names
IDH, IDP, IDHM, IDPM, ICD-M, D2HGA2, mNADP-IDHICD-M, IDH IDH2 D2HGA2, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-IDH isocitrate dehydrogenase (NADP(+)) 2 isocitrate dehydrogenase [NADP], mitochondrial|NADP(+)-specific ICDH|isocitrate dehydrogenase (NADP(+)) 2, mitochondrial|isocitrate dehydrogenase 2 (NADP+), mitochondrial|oxalosuccinate decarboxylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IDH2, check out the IDH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IDH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IDH2 Antibody (A00510)
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