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Product Info Summary
| SKU: | PB9495 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ICA1 Antibody Picoband®
SKU/Catalog Number
PB9495
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ICA1 Antibody Picoband® catalog # PB9495. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ICA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9495)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ICA1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9495 is reactive to ICA1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60-65 kDa
Calculated molecular weight
54645 MW
Background of ICA1
Islet cell autoantigen 1 is a protein that in humans is encoded by the ICA1 gene. It is mapped to 7p22. This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. What’s more, this protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Several transcript variants encoding two different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9495 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human SH-SY5Y whole cell, human RT4 whole cell, human MCF-7 whole cell, human K562 whole cell, rat pancreas tissue, rat brain tissue, mouse pancreas tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ICA1 using anti-ICA1 antibody (PB9495).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat pancreas tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse pancreas tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICA1 antigen affinity purified polyclonal antibody (Catalog # PB9495) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICA1 at approximately 60-65 kDa. The expected band size for ICA1 is at 55 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ICA1 (Source: Uniprot.org, NCBI)
Gene Name
ICA1
Full Name
Islet cell autoantigen 1
Weight
54645 MW
Alternative Names
Islet cell autoantigen 1;69 kDa islet cell autoantigen;ICA69;Islet cell autoantigen p69;ICAp69;p69;ICA1; ICA1 ICA69, ICAp69 islet cell auto 1 islet cell auto 1|69 kDa islet cell auto|diabetes mellitus type I auto|islet cell auto 1, 69kDa|islet cell auto p69|p69|testicular tissue protein Li 162
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ICA1, check out the ICA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ICA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ICA1 Antibody Picoband® (PB9495)
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Customer Q&As
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1 Customer Q&As for Anti-ICA1 Antibody Picoband®
Question
We are currently using anti-ICA1 antibody PB9495 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The anti-ICA1 antibody (PB9495) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-17
