Product Info Summary
SKU: | A09580-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-IARS2 Antibody Picoband™
SKU/Catalog Number
A09580-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-IARS2 Antibody Picoband™ catalog # A09580-2. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-IARS2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09580-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human OTUB1 recombinant protein (Position: K10-K271). Human OTUB1 shares 99.6% amino acid (aa) sequence identity with both mouse and rat OTUB1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09580-2 is reactive to IARS2 in Human, Mouse, Rat
Applications
A09580-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
114 kDa
Calculated molecular weight
52588 MW
Background of IARS2
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Immunohistochemistry, 2-5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a09580 2 iars2 primary antibodies wb testing 1 a09580 2 iars2 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a09580-2-iars2-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of IARS2 using anti-IARS2 antibody (A09580-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: human Hacat whole cell lysates,
Lane 6: human HepG2 whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: rat kidney tissue lysates,
Lane 9: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IARS2 antigen affinity purified polyclonal antibody (Catalog # A09580-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IARS2 at approximately 114 kDa. The expected band size for IARS2 is at 114 kDa.
![a09580 2 iars2 primary antibodies ihc testing 2 a09580 2 iars2 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a09580-2-iars2-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of IARS2 using anti-IARS2 antibody (A09580-2).
IARS2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IARS2 Antibody (A09580-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a09580 2 iars2 primary antibodies ihc testing 3 a09580 2 iars2 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a09580-2-iars2-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of IARS2 using anti-IARS2 antibody (A09580-2).
IARS2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IARS2 Antibody (A09580-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a09580 2 iars2 primary antibodies ihc testing 4 a09580 2 iars2 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a09580-2-iars2-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of IARS2 using anti-IARS2 antibody (A09580-2).
IARS2 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IARS2 Antibody (A09580-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a09580 2 iars2 primary antibodies ihc testing 5 a09580 2 iars2 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a09580-2-iars2-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of IARS2 using anti-IARS2 antibody (A09580-2).
IARS2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IARS2 Antibody (A09580-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a09580 2 iars2 primary antibodies ihc testing 6 a09580 2 iars2 primary antibodies ihc testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a09580-2-iars2-primary-antibodies-ihc-testing-6.jpg)
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Figure 6. IHC analysis of IARS2 using anti-IARS2 antibody (A09580-2).
IARS2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IARS2 Antibody (A09580-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a09580 2 iars2 primary antibodies fcm testing 7 a09580 2 iars2 primary antibodies fcm testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a09580-2-iars2-primary-antibodies-fcm-testing-7.png)
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Figure 7. Flow Cytometry analysis of 293T cells using anti-IARS2 antibody (A09580-2).
Overlay histogram showing 293T cells stained with A09580-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IARS2 Antibody (A09580-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For IARS2 (Source: Uniprot.org, NCBI)
Gene Name
IARS2
Full Name
Isoleucine--tRNA ligase, mitochondrial
Weight
52588 MW
Superfamily
class-I aminoacyl-tRNA synthetase family
Alternative Names
EC 6.1.1; EC 6.1.1.5; FLJ10326; ILERS; isoleucine tRNA ligase 2, mitochondrial; Isoleucine--tRNA ligase; isoleucine-tRNA synthetase 2, mitochondrial; isoleucyl-tRNA synthetase 2, mitochondrial; isoleucyl-tRNA synthetase, mitochondrial; mitochondrial isoleucine tRNA synthetase IARS2 CAGSSS, ILERS isoleucyl-tRNA synthetase 2, mitochondrial isoleucine--tRNA ligase, mitochondrial|isoleucine tRNA ligase 2, mitochondrial|isoleucine-tRNA synthetase 2, mitochondrial|isoleucyl-tRNA synthetase, mitochondrial|mitochondrial isoleucine tRNA synthetase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IARS2, check out the IARS2 Infographic
![IARS2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IARS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IARS2 Antibody Picoband™ (A09580-2)
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