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Product Info Summary
| SKU: | M00736 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HuR / ELAVL1 Monoclonal Antibody
View all HuR/ELAVL1 Antibodies
SKU/Catalog Number
M00736
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HuR / ELAVL1 Monoclonal Antibody catalog # M00736. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HuR / ELAVL1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00736)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADHI-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HuR / ELAVL1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00736 is reactive to ELAVL1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
36 kDa
Calculated molecular weight
85941 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00736 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
FC 1:50
Positive Control
WB: human Jurkat whole cell, human Hela whole cell, human MOLT-4 whole cell, human HepG2 whole cell, rat heart tissue, rat spleen tissue, mouse heart tissue, mouse spleen tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ELAVL1 using anti-ELAVL1 antibody (M00736).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELAVL1 antigen affinity purified monoclonal antibody (Catalog # M00736) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELAVL1 at approximately 36 kDa. The expected band size for ELAVL1 is at 36 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ELAVL1 (Source: Uniprot.org, NCBI)
Gene Name
ELAVL1
Full Name
ELAV-like protein 1
Weight
85941 MW
Superfamily
RRM elav family
Alternative Names
Signal transducer and activator of transcription 4;STAT4; ELAVL1 ELAV1, HUR, Hua, MelG ELAV like RNA binding protein 1 ELAV-like protein 1|ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu R)|Hu R|embryonic lethal, abnormal vision, drosophila, homolog-like 1|hu- R
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ELAVL1, check out the ELAVL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ELAVL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HuR / ELAVL1 Monoclonal Antibody (M00736)
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No publications found for M00736
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Customer Q&As
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5 Customer Q&As for Anti-HuR / ELAVL1 Monoclonal Antibody
Question
I was wanting to use your anti-HuR / ELAVL1 Monoclonal antibody for IHC for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
It shows on the product datasheet, M00736 anti-HuR / ELAVL1 Monoclonal antibody has been tested for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-15
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-HuR / ELAVL1 Monoclonal antibody M00736. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-27
Question
We are interested in to test anti-HuR / ELAVL1 Monoclonal antibody M00736 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-HuR / ELAVL1 Monoclonal antibody M00736 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Krishna
Verified customer
Asked: 2019-08-07
Answer
The products we sell, including anti-HuR / ELAVL1 Monoclonal antibody M00736, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-07
Question
We are currently using anti-HuR / ELAVL1 Monoclonal antibody M00736 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
The anti-HuR / ELAVL1 Monoclonal antibody (M00736) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-11
Question
Is this M00736 anti-HuR / ELAVL1 Monoclonal antibody reactive to the isotypes of ELAVL1?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
The immunogen of M00736 anti-HuR / ELAVL1 Monoclonal antibody is A synthesized peptide derived from human HuR / ELAVL1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-26
