Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | M07514-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, ICC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124
SKU/Catalog Number
M07514-2
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124 (Catalog # M07514-2). Tested in ELISA, ICC, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07514-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM124
Isotype
Rabbit IgG
Immunogen
Human IgA
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human IgA1, and very slightly cross reacts with IgA2. No cross-reactivity with human IgG, IgM, IgD, or IgE.
Reactive Species
M07514-2 is reactive to IGHA1 in Human
Reconstitution
Calculated molecular weight
37655 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07514-2 is guaranteed for ELISA, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection)
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.1ug/mL-1ug/mL.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC result
Immunohistochemistry of human tonsil tissue using anti-human IgA1 antibody RM124
Click image to see more details
Figure 2. ELISA result showing specificity
Sandwich ELISA using RM124 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Click image to see more details
Figure 3. ELISA result showing specificity
Sandwich ELISA using RM124 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Click image to see more details
Figure 4. ELISA result showing specificity
ELISA of human immunoglobulins shows RM124 only reacted to human IgA1. Very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM124 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Click image to see more details
Figure 5. ELISA result showing specificity
A titer ELISA using RM124. The plate was coated with different amounts of human IgA1. A serial dilution of RM124 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For IGHA1 (Source: Uniprot.org, NCBI)
Gene Name
IGHA1
Full Name
Immunoglobulin heavy constant alpha 1
Weight
37655 MW
Alternative Names
Immunoglobulin heavy constant alpha 1 ;Ig alpha-1 chain C region ;Ig alpha-1 chain C region BUR ;Ig alpha-1 chain C region TRO ;IGHA1 ; IGHA1 IgA1 immunoglobulin heavy constant alpha 1 immunoglobulin alpha 1|immunoglobulin heavy chain constant region alpha 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGHA1, check out the IGHA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGHA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124 (M07514-2)
Hello CJ!
M07514-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Quantitative Proteomic Analysis of Escherichia coli Heat-Labile Toxin B Subunit (LTB) with Enterovirus 71 (EV71) Subunit VP1
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124
Question
We are currently using anti-Human IgA1 Rabbit Monoclonal antibody, Clone#RM124 M07514-2 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
The anti-Human IgA1 Rabbit Monoclonal antibody, Clone#RM124 (M07514-2) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-11
