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Product Info Summary
| SKU: | RP1011 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-D-dopachrome decarboxylase DDT Antibody Picoband®
SKU/Catalog Number
RP1011
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-D-dopachrome decarboxylase DDT Antibody catalog # RP1011. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-D-dopachrome decarboxylase DDT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1011)
Host
Rabbit
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human DDT recombinant protein (Position: M1-L118).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1011 is reactive to DDT in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
13-15 kDa
Calculated molecular weight
12712 MW
Background of DDT
DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1011 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA, 0.1-0.5μg/ml, -,
Positive Control
WB: human hepatocellular carcinoma tumor tissue (HCCT), rat liver tissue, mouse liver tissue
IHC: human placenta tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DDT using anti-DDT antibody (RP1011, Left) and anti-DDT antibody (PA1449, Right).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDT antigen affinity purified polyclonal antibody (Catalog # RP1011) and rabbit anti-DDT antigen affinity purified polyclonal antibody (Catalog # PA1449) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDT at approximately 13-15 kDa. The expected band size for DDT is at 13 kDa.
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Figure 2. IHC analysis of DDT using anti-DDT antibody (RP1011).
DDT was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDT Antibody (RP1011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For DDT (Source: Uniprot.org, NCBI)
Gene Name
DDT
Full Name
D-dopachrome decarboxylase
Weight
12712 MW
Superfamily
MIF family
Alternative Names
D-dopachrome decarboxylase;4.1.1.84;D-dopachrome tautomerase;Phenylpyruvate tautomerase II;DDT; DDT D-DT, DDCT, MIF-2, MIF2 D-dopachrome tautomerase D-dopachrome decarboxylase|phenylpyruvate tautomerase II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDT, check out the DDT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-D-dopachrome decarboxylase DDT Antibody Picoband® (RP1011)
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Customer Q&As
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7 Customer Q&As for Anti-D-dopachrome decarboxylase DDT Antibody Picoband®
Question
Is this RP1011 anti-DDT antibody reactive to the isotypes of DDT?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
The immunogen of RP1011 anti-DDT antibody is E. coli-derived human DDT recombinant protein(Position: M1-L118). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-04
Question
Is there a BSA free version of anti-DDT antibody RP1011 available?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DDT antibody RP1011 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-17
Question
We are currently using anti-DDT antibody RP1011 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The anti-DDT antibody (RP1011) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-28
Question
Please see the WB image, lot number and protocol we used for placenta using anti-DDT antibody RP1011. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-30
Question
I have a question about product RP1011, anti-DDT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1011 anti-DDT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-23
Question
We are interested in to test anti-DDT antibody RP1011 on human placenta for research purposes, then I may be interested in using anti-DDT antibody RP1011 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-30
Answer
The products we sell, including anti-DDT antibody RP1011, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-30
Question
Does RP1011 anti-DDT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Gonzalez
Verified customer
Asked: 2016-04-26
Answer
It shows on the product datasheet, RP1011 anti-DDT antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-04-26
