Click image to see more details
Product Info Summary
| SKU: | FC00591-PE |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD71 TFRC Monoclonal Antibody PE Conjugated, Flow Validated
SKU/Catalog Number
FC00591-PE
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD71 TFRC Monoclonal Antibody PE Conjugated, Flow Validated (Catalog# FC00591-PE). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD71 TFRC Monoclonal Antibody PE Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00591-PE)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: OKT9
Isotype
IgG1,k
Immunogen
Human acute lymphoblastic leukaemia cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00591-PE is reactive to TFRC in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
84871 MW
Background of TFRC
CD71 is also known as transferrin receptor (TFRC). Nikinmaa and Schroder (1987) concluded that p90 and TFRC are the same protein: studies using monoclonal antibodies indicated that exhaustive precipitation of radioactively labeled lysates with one antibody removed all activity of lysates with the other. Peptide maps of antigens recognized with both antibodies showed great similarity and indicated that both antibodies react with the same antigen, the human transferrin receptor, but with different antigenic sites of the molecule.TFR is assigned to chromosome 3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00591-PE is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Jurkat E6.1 cell lines stained with anti-human CD71 PE (clone OKT9) (red histogram). Jurkat E6.1 cell lines stained with IgG1 MOPC-31C PE isotype control (blue histogram)
Protein Target Info & Infographic
Gene/Protein Information For TFRC (Source: Uniprot.org, NCBI)
Gene Name
TFRC
Full Name
Transferrin receptor protein 1
Weight
84871 MW
Superfamily
peptidase M28 family
Alternative Names
Transferrin receptor protein 1;TR;TfR;TfR1;Trfr;T9;p90;CD71;Transferrin receptor protein 1, serum form;sTfR;TFRC; TFRC CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90 transferrin receptor transferrin receptor protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TFRC, check out the TFRC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TFRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD71 TFRC Monoclonal Antibody PE Conjugated, Flow Validated (FC00591-PE)
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No publications found for FC00591-PE
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-human CD71 TFRC Monoclonal Antibody PE Conjugated, Flow Validated
Question
Please see the WB image, lot number and protocol we used for melanoma using anti-human CD71 Monoclonal antibody PE Conjugated, Flow Validated FC00591-PE. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma using anti-human CD71 Monoclonal antibody PE Conjugated, Flow Validated FC00591-PE. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-24
Question
I see that the anti-human CD71 Monoclonal antibody PE Conjugated, Flow Validated FC00591-PE works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-27
Question
Does anti-human CD71 Monoclonal antibody PE Conjugated, Flow Validated FC00591-PE work for Flow Cytometry with melanoma?
S. Zhao
Verified customer
Asked: 2018-04-18
Answer
According to the expression profile of melanoma, TFRC is highly expressed in melanoma. So, it is likely that anti-human CD71 Monoclonal antibody PE Conjugated, Flow Validated FC00591-PE will work for Flow Cytometry with melanoma.
Boster Scientific Support
Answered: 2018-04-18
Question
Can you help my question with product FC00591-PE, anti-human CD71 Monoclonal antibody PE Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Jones
Verified customer
Asked: 2014-12-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00591-PE anti-human CD71 Monoclonal antibody PE Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-12-03
Question
Does FC00591-PE anti-human CD71 Monoclonal antibody PE Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Edwards
Verified customer
Asked: 2013-09-30
Answer
As indicated on the product datasheet, FC00591-PE anti-human CD71 Monoclonal antibody PE Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-09-30
Question
Is a blocking peptide available for product anti-human CD71 Monoclonal antibody PE Conjugated, Flow Validated (FC00591-PE)?
A. Krishna
Verified customer
Asked: 2013-07-26
Answer
We do provide the blocking peptide for product anti-human CD71 Monoclonal antibody PE Conjugated, Flow Validated (FC00591-PE). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-07-26
