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Product Info Summary
| SKU: | FC00052 |
|---|---|
| Size: | 100ug, 500ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD44 Monoclonal Antibody, Flow Validated
SKU/Catalog Number
FC00052
Size
100ug, 500ug
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human CD44 Monoclonal Antibody Unconjugated Conjugated, Flow Validated (Catalog# FC00052). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human CD44 Monoclonal Antibody, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00052)
Host
Mouse
Contents
PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: Hermes-3
Isotype
IgG2a,k
Immunogen
CD44 recombinant protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00052 is reactive to CD44 in Human
Reconstitution
Calculated molecular weight
81538 MW
Background of CD44
CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene.CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1. CD45 is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00052 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Lymphocytes gated PBMCs stained with purified anti-human CD44 (clone Hermes-3) followed by goat anti-mouse IgG (H+L) Alexa Fluor 488 (red histogram). Lymphocytes gated PBMCs stained with mouse IgG2a isotype control followed by goat anti-mouse IgG (H+L) Alexa Fluor 488 (Blue histogram). The data are generated by BD Accuri C6 Flow Cytometer and analyzed by FlowJo software.
Protein Target Info & Infographic
Gene/Protein Information For CD44 (Source: Uniprot.org, NCBI)
Gene Name
CD44
Full Name
CD44 antigen
Weight
81538 MW
Alternative Names
CD44 antigen;CDw44;Epican;Extracellular matrix receptor III;ECMR-III;GP90 lymphocyte homing/adhesion receptor;HUTCH-I;Heparan sulfate proteoglycan;Hermes antigen;Hyaluronate receptor;Phagocytic glycoprotein 1;PGP-1;Phagocytic glycoprotein I;PGP-I;CD44;CD44;LHR, MDU2, MDU3, MIC4; CD44 CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1 CD44 molecule (Indian blood group) CD44 |GP90 lymphocyte homing/adhesion receptor|Hermes |Indian blood group |cell surface glycoprotein CD44|chondroitin sulfate proteoglycan 8|epican|extracellular matrix receptor III|hematopoietic cell E- and L-selectin ligand|heparan sulfate proteoglycan|homing function and Indian blood group system|hyaluronate receptor|phagocytic glycoprotein 1|soluble CD44
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD44, check out the CD44 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD44: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD44 Monoclonal Antibody, Flow Validated (FC00052)
Hello CJ!
FC00052 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang GF,Wu JC,Wang HY,Jiang WD,Qiu L. Overexpression of microRNA-205-5p exerts suppressive effects on stem cell drug resistance in gallbladder cancer by down-regulating PRKCE. Biosci Rep.2020 Sep 30;40(9): BSR20194509.doi:10.1042/BSR20194509.PMID: 328698
Species: Human,Mouse
FC00052 usage in article: APP:FLOW CYTOMETRY, SAMPLE:GBC STEM CELLS, DILUTION:NA
Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma
Rat nasal respiratory mucosa-derived ectomesenchymal stem cells differentiate into Schwann-like cells promoting the differentiation of PC12 cells and %u2026
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Anti-Human CD44 Monoclonal Antibody, Flow Validated
Question
Here is the WB image, lot number and protocol we used for glial tumor using anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC00052. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-01
Question
I have a question about product FC00052, anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00052 anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-19
Question
Would FC00052 anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Carter
Verified customer
Asked: 2019-11-26
Answer
It shows on the product datasheet, FC00052 anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-26
Question
Do you have a BSA free version of anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC00052 available?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC00052 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-07
Question
Will anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC00052 work for Flow Cytometry with glial tumor?
V. Miller
Verified customer
Asked: 2019-07-16
Answer
According to the expression profile of glial tumor, CD44 is highly expressed in glial tumor. So, it is likely that anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC00052 will work for Flow Cytometry with glial tumor.
Boster Scientific Support
Answered: 2019-07-16
Question
Is this FC00052 anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated reactive to the isotypes of CD44?
B. Bhatt
Verified customer
Asked: 2019-04-03
Answer
The immunogen of FC00052 anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated is CD44 recombinant protein . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-03
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for glial tumor using anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC00052. Let me know if you need anything else.
P. Roberts
Verified customer
Asked: 2018-03-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-23
Question
I was wanting to use your anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated for Flow Cytometry for human glial tumor on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human glial tumor identification?
T. Wu
Verified customer
Asked: 2016-04-25
Answer
As indicated on the product datasheet, FC00052 anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human glial tumor in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-04-25
Question
Our lab want to know about to test anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC00052 on human glial tumor for research purposes, then I may be interested in using anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC00052 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Williams
Verified customer
Asked: 2014-12-19
Answer
The products we sell, including anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC00052, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-12-19
Question
Is a blocking peptide available for product anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated (FC00052)?
A. Johnson
Verified customer
Asked: 2014-03-31
Answer
We do provide the blocking peptide for product anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated (FC00052). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-03-31
Question
I see that the anti-Human CD44 Monoclonal antibody Unconjugated Conjugated, Flow Validated FC00052 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
D. Krishna
Verified customer
Asked: 2013-11-07
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-11-07
