Anti-Human CD3 Monoclonal Antibody iFluor647 Conjugated, Flow Validated

SKU FC02675-iFluor647
Size 25 Tests, 100 Tests, 200 Tests
Reactivity Human
Clonality Monoclonal
Clone Number UCHT1
Host Mouse
Applications Flow Cytometry

Overview

Product Name Anti-Human CD3 Monoclonal Antibody iFluor647 Conjugated, Flow Validated
SKU/Catalog Number FC02675-iFluor647
Size 25 Tests, 100 Tests, 200 Tests
Description Anti-Human CD3 Monoclonal Antibody iFluor647 Conjugated, Flow Validated
Cite This Product Anti-Human CD3 Monoclonal Antibody iFluor647 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC02675-iFluor647)
Host Mouse
Contents/Buffer PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Form Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Immunogen Human peripheral blood lymphocytes
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Flow Cytometry

Images And Assay Conditions

Lymphocytes gated healthy volunteer blood stained with iFluor647 conjugated anti-human CD3 (clone UCHT1) before RBC lysis (red histogram). Lymphocytes gated RBC lysed healthy volunteer blood FL-4 FMO control (Blue histogram). The flow data are generated i

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P07766
Gene Name CD3E
Protein Name T-cell surface glycoprotein CD3 epsilon chain
Alternative Names T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;CD3E;T3E;
Subcellular Localization Cell membrane ; Single-pass type I membrane protein .
Molecular Weight 23147 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell developement. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down- regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). .
Research Areas Human

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In stock
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FC02675-iFluor647
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$90.00

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Publications

Li Y, Geng S, Yin Q, Chen S, Yang L, Wu X, Li B, Du X, Schmidt Ca, Przybylski Gk. J Transl Med. 2010 May 14;8:47. Doi: 10.1186/1479-5876-8-47. Decreased Level Of Recent Thymic Emigrants In Cd4+ And Cd8+T Cells From Cml Patients.
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