Anti-human CD235a GYPA Monoclonal Antibody FITC Conjugated, Flow Validated

Product Name

Anti-human CD235a GYPA Monoclonal Antibody FITC Conjugated, Flow Validated

View all Glycophorin A Antibodies

SKU/Catalog Number

FC02184-FITC

Size

100ug

Form

Liquid

Description

Boster Bio Anti-human CD235a GYPA Monoclonal Antibody FITC Conjugated, Flow Validated (Catalog# FC02184-FITC). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human CD235a GYPA Monoclonal Antibody FITC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC02184-FITC)

Host

Mouse

Contents

PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: GYPA280

Isotype

IgG1,k

Immunogen

Recombinant human glycophorin A protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC02184-FITC is reactive to GYPA in Human

Applications

FC02184-FITC is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

16331 MW

Background of Glycophorin A

Glycophorin A (MNS blood group), also known as GYPA, is a protein which in humans is encoded by the GYPA gene. GYPA has also recently been designated CD235a (cluster of differentiation 235a). Glycophorins A (GYPA; this protein) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

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RBCs gated human blood stained with anti-human CD235a FITC (clone GYPA/280, red histogram). Blue histogram is for the RBCs gated stained whole blood with IgG1 (MOPC31) FITC conjugated isotype control.

Gene/Protein Information For GYPA (Source: Uniprot.org, NCBI)

Alternative Names

CD235a antigen; CD235a; glycophorin A (includes MN blood group); glycophorin A (MNS blood group); Glycophorin A; glycophorin Erik; glycophorin MiI; glycophorin MiIII; glycophorin MiV; glycophorin MiX; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GpMiIII; GPSAT; GYPA; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); Mi.V glycoprotein (24 AA); MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR GYPA CD235a, GPA, GPErik, GPSAT, HGpMiV, HGpMiXI, HGpSta(C), MN, MNS, PAS-2 glycophorin A (MNS blood group) glycophorin-A|MN sialoglycoprotein|Mi.V glycoprotein (24 AA)|erythroid-lineage-specific membrane sialoglycoprotein|glycophorin A (MN blood group)|glycophorin A, GPA|glycophorin Erik|glycophorin MiI|glycophorin MiV|glycophorin SAT|glycophorin Sta type C|recombinant glycophorin A-B Miltenberger-DR|sialoglycoprotein alpha

For more info on GYPA, check out the GYPA Infographic

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In this infographic, you will see the following information for GYPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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