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Product Info Summary
| SKU: | M02073 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IP, ICC, WB |
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Product info
Product Name
Anti-Hu CD42b Purified GP1BA Monoclonal Antibody
View all CD42b/GPIb alpha Antibodies
SKU/Catalog Number
M02073
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD42b Purified GP1BA Monoclonal Antibody (Catalog# M02073). Tested in Flow Cytometry, IP, WB, ICC, ELISA application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD42b Purified GP1BA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02073)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
AK2
Isotype
Mouse IgG1
Immunogen
Human platelets. The mouse monoclonal antibody AK2 recognizes an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02073 is reactive to GP1BA in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
71540 MW
Background of CD42b/GPIb alpha
CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02073 is guaranteed for ELISA, Flow Cytometry, IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry validation of GP1BA using Anti-Hu CD42b Purified GP1BA Monoclonal Antibody (M02073).
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD42b (AK2) purified antibody (concentration in sample 4 μg/ml) GAM APC.
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Figure 2. Flow Cytometry validation of GP1BA using Anti-Hu CD42b Purified GP1BA Monoclonal Antibody (M02073).
Separation of human thrombocytes (red-filled) from CD42b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD42b (AK2) purified antibody (concentration in sample 4 μg/ml) GAM APC.Protein Target Info & Infographic
Gene/Protein Information For GP1BA (Source: Uniprot.org, NCBI)
Gene Name
GP1BA
Full Name
Platelet glycoprotein Ib alpha chain
Weight
71540 MW
Alternative Names
BSS, GP1B, VWDP, CD42B, GPIbA, BDPLT1, BDPLT3, DBPLT3 GP1BA BDPLT1, BDPLT3, BSS, CD42B, CD42b-alpha, DBPLT3, GP1B, GPIbA, GPIbalpha, VWDP glycoprotein Ib platelet subunit alpha platelet glycoprotein Ib alpha chain|GP-Ib alpha| CD42b-alpha|glycoprotein Ib (platelet), alpha polypeptide|glycoprotein Ib platelet alpha subunit|mutant platelet membrane glycoprotein Ib-alpha|platelet membrane glycoprotein 1b-alpha subunit|platelet membrane glycoprotein Ib-alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GP1BA, check out the GP1BA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GP1BA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD42b Purified GP1BA Monoclonal Antibody (M02073)
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