Anti-Hu CD164 Purified Monoclonal Antibody

Product Name

Anti-Hu CD164 Purified Monoclonal Antibody

View all CD164 Antibodies

SKU/Catalog Number

M05879

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD164 Purified Monoclonal Antibody (Catalog# M05879). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD164 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05879)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

67D2

Isotype

Mouse IgG1

Immunogen

Breast tumor cell line T-47D. The mouse monoclonal antibody 67D2 recognizes an extracellular class III epitope (not sensitive to sialidase, N-glycanase, O-glycosidase, and O-sialoglycoprotease) of CD164, a sialomucin expressed in hematopoietic myeloid and erythroid progenitors, activated basophils, and in various carcinomas and leukemic cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Cross-reactivity with CLIC5b was not determined.

Reactive Species

M05879 is reactive to CD164 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

20917 MW

Background of CD164

CD164, also known as endolyn, is a type I transmembrane protein with heavily glycosylated extracellular part containing sialic acid and glycosaminoglycan residues. CD164 plays both adhesive and antiadhesive role and serves as a potent negative regulator for CD34+ CD38- hematopoietic progenitor cell proliferation. It has also been reported to be involved in myogenic differentiation and cancer metastasis. The adhesive and negative regulatory functions seem to depend on different posttranslational modifications of CD164 protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M05879 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

Gene/Protein Information For CD164 (Source: Uniprot.org, NCBI)

Alternative Names

DFNA66, MUC-24, MGC-24, endolyn CD164 DFNA66, MGC-24, MGC-24v, MUC-24, endolyn CD164 molecule sialomucin core protein 24|CD164 , sialomucin|multi-glycosylated core protein 24

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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