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Product Info Summary
| SKU: | M01767-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, ICC, WB |
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Product info
Product Name
Anti-Hu CD133 Purified PROM1 Monoclonal Antibody
SKU/Catalog Number
M01767-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD133 Purified PROM1 Monoclonal Antibody (Catalog# M01767-1). Tested in Flow Cytometry, WB, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD133 Purified PROM1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01767-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
293C3
Isotype
Mouse IgG2b
Immunogen
Human CD133 transfectants. The mouse monoclonal antibody 293C3 recognizes the extracellular epitope 2 on human CD133 (CD133/2), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of human renal progenitors.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01767-1 is reactive to PROM1 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
97202 MW
Background of CD133
CD133 (prominin 1) is a 5-transmembrane glycoprotein with extracellular N- and intracellular C-terminus. CD133 function remains to be elucidated, but it can be used as a cancer stem cell marker. Its expression pattern in progenitor cells is similar to CD34, i.e. on hematopoietic stem cells in bone marrow, cord blood, neural stem cells, retinoblastoma, or endothelial precursor cells (not mature endothelial cells). It is being used for identification and isolation of hematopoietic stem cells, including isolation for stem cell transplantation. Expression of CD133 correlates with differentiation of human colon cancer cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01767-1 is guaranteed for Flow Cytometry, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry: Formaldehyde fixation can be used.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry validation of PROM1 using Anti-Hu CD133 Purified PROM1 Monoclonal Antibody (M01767-1).
Flow cytometry analysis (surface staining) of CD133 in human peripheral blood with anti-CD133 (293C3) purifiedProtein Target Info & Infographic
Gene/Protein Information For PROM1 (Source: Uniprot.org, NCBI)
Gene Name
PROM1
Full Name
Prominin-1
Weight
97202 MW
Superfamily
prominin family
Alternative Names
Prominin 1, PROM1, AC133, PROML1, STGD4 PROM1 AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4 prominin 1 prominin-1| AC133|hProminin|hematopoietic stem cell |prominin-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PROM1, check out the PROM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PROM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD133 Purified PROM1 Monoclonal Antibody (M01767-1)
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