Anti-Hu CD111 Purified NECTIN1 Monoclonal Antibody

Product Name

Anti-Hu CD111 Purified NECTIN1 Monoclonal Antibody

View all Nectin-1/PVRL1 Antibodies

SKU/Catalog Number

M04988

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD111 Purified NECTIN1 Monoclonal Antibody (Catalog# M04988). Tested in Flow Cytometry, IP, WB, IHC, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD111 Purified NECTIN1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04988)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

R1.302

Isotype

Mouse IgG1 kappa

Immunogen

NIH/3T3 cells transfected with human CD111. The mouse monoclonal antibody R1.302 recognizes an extracellular epitope on CD111 (also known as Nectin 1), a 75 kDa type I transmembrane glycoprotein broadly expressed on endothelial cells, epithelial cells, neuronal cells, megakaryocytes, and CD34-positive stem cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M04988 is reactive to NECTIN1 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

57158 MW

Background of Nectin-1/PVRL1

CD111, also known as nectin-1, is a calcium-independent cell-cell adhesion transmembrane glycoprotein involved in organization of adherens junctions and tight junctions in epithelial and endothelial cells. It also serves as a target molecule for entry of herpes simplex virus (HSV-1, HSV-2) and pseudorabies virus (PRV) into epithelial and neuronal cells. CD111 is connected with actin cytoskeleton through afadin. Mutations in the gene for CD111 cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M04988 is guaranteed for Flow Cytometry, IP, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

Gene/Protein Information For NECTIN1 (Source: Uniprot.org, NCBI)

Alternative Names

NECTIN1, ED4, HIgR, HV1S, HVEC, OFC7, PRR1, PVRR, CD111, PVRL1, PVRR1, SK-12, CLPED1 NECTIN1 CD111, CLPED1, ED4, HIgR, HV1S, HVEC, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK-12, nectin-1 nectin cell adhesion molecule 1 nectin-1|ectodermal dysplasia 4 (Margarita Island type)|herpes simplex virus type 1 sensitivity|herpes virus entry mediator C|herpesvirus Ig-like receptor|nectin 1|poliovirus receptor-like 1|poliovirus receptor-related 1 (herpesvirus entry mediator C)|poliovirus receptor-related protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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