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Key=contents, value=Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Key=background
Key=gene_name, value=HSP90AA1
Key=synonyms, value=Heat shock protein HSP 90-alpha;Heat shock 86 kDa;HSP 86;HSP86;Lipopolysaccharide-associated protein 2;LAP-2;LPS-associated protein 2;Renal carcinoma antigen NY-REN-38;HSP90AA1;HSP90A, HSPC1, HSPCA;
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ICC/IF 1:50-1:200
IP 1:50
Key=subcellular_localization, value= Cytoplasm. Melanosome. Cell membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Key=tissue_specificity, value= Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U-937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
Key=protein_function, value= Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. .
Key=application_notes, value=Specific for endogenous levels of the ~100 kDa GluR1 protein phosphorylated at Ser845. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
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Key=immunogen, value=A synthesized peptide derived from human Hsp90 alpha
Key=purification, value=Affinity-chromatography
Key=storage, value=Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
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ICC/IF: Hela cell
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Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody

HSP90AA1 antibody

Boster Bio Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody catalog # M01103-3. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M01103-3
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, ICC, WB

Product Name

Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody

View all HSP90AA1 Antibodies

SKU/Catalog Number

M01103-3

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody catalog # M01103-3. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01103-3)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BAC-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Hsp90 alpha

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01103-3 is reactive to HSP90AA1 in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

80-100 kDa

Calculated molecular weight

84660 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M01103-3 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50

Positive Control

WB: PC-12 cell, HeLa cell
ICC/IF: Hela cell

Validation Images & Assay Conditions

Gene/Protein Information For HSP90AA1 (Source: Uniprot.org, NCBI)

Gene Name

HSP90AA1

Full Name

Heat shock protein HSP 90-alpha

Weight

84660 MW

Superfamily

heat shock protein 90 family

Alternative Names

Heat shock protein HSP 90-alpha;Heat shock 86 kDa;HSP 86;HSP86;Lipopolysaccharide-associated protein 2;LAP-2;LPS-associated protein 2;Renal carcinoma antigen NY-REN-38;HSP90AA1;HSP90A, HSPC1, HSPCA; HSP90AA1 EL52, HEL-S-65p, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp103, Hsp89, Hsp90, LAP-2, LAP2 heat shock protein 90 alpha family class A member 1 heat shock protein HSP 90-alpha|HSP 86|LPS-associated protein 2|epididymis luminal secretory protein 52|epididymis secretory sperm binding protein Li 65p|heat shock 86 kDa|heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|heat shock 90kD protein, alpha-like 4|heat shock 90kDa protein 1, alpha|heat shock protein 90kDa alpha (cytosolic), class A member 1|heat shock protein 90kDa alpha family class A member 1|lipopolysaccharide-associated protein 2|renal carcinoma NY-REN-38

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HSP90AA1, check out the HSP90AA1 Infographic

HSP90AA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HSP90AA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01103-3 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Youwen Zhang,Junye Wang,Shucheng Ye et al.Co-overexpression of STIP1 and Hsp90 correlates with progression and prognosis of lung adenocarcinoma,29 January 2020,PREPRINT (Version 2) available at Research Square [https://doi.org/10.21203/rs.2.13680/v2]
Species: Human
M01103-3 usage in article: APP:IHC, SAMPLE:LAC TISSUE, DILUTION:1:300

Jiang Q, Wang Y, Li T, Shi K, Li Z, Ma Y, Li F, Luo H, Yang Y, Xu C. Mol Biol Cell. 2011 Apr 15;22(8):1167-80. Doi: 10.1091/Mbc.E10-10-0860. Epub 2011 Feb 23. Heat Shock Protein 90-Mediated Inactivation Of Nuclear Factor-??b Switches Autophagy To ...

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4 Customer Q&As for Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody

Question

My boss were content with the WB result of your anti-Hsp90 alpha Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma nucleus. using this antibody. Is that expected? Could you tell me where is HSP90AA1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

According to literature, cervix carcinoma does express HSP90AA1. Generally HSP90AA1 expresses in nucleus. Regarding which tissues have HSP90AA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 2492519, 10409742
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 18318008, 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Peripheral blood lymphocyte, Pubmed ID: 2780322
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 2527334, 15489334
Placenta, and Teratocarcinoma, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Renal cell carcinoma, Pubmed ID: 10508479, 11274138
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2020-03-03

Question

We have observed staining in rat placenta. What should we do? Is anti-Hsp90 alpha Rabbit Monoclonal antibody supposed to stain placenta positively?

Verified Customer

Verified customer

Asked: 2018-10-12

Answer

Based on literature placenta does express HSP90AA1. Based on Uniprot.org, HSP90AA1 is expressed in middle temporal gyrus, peripheral blood lymphocyte, placenta, placenta teratocarcinoma, brain, cajal-retzius cell fetal brain cortex, heart, renal cell carcinoma, lymphoblast, cervix carcinoma, pituitary, t-cell, platelet, liver, leukemic t-cell, melanoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have HSP90AA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 2492519, 10409742
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 18318008, 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Peripheral blood lymphocyte, Pubmed ID: 2780322
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 2527334, 15489334
Placenta, and Teratocarcinoma, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Renal cell carcinoma, Pubmed ID: 10508479, 11274138
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2018-10-12

Question

We are currently using anti-Hsp90 alpha Rabbit Monoclonal antibody M01103-3 for rat tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?

D. Miller

Verified customer

Asked: 2016-01-07

Answer

The anti-Hsp90 alpha Rabbit Monoclonal antibody (M01103-3) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-01-07

Question

Our lab used your anti-Hsp90 alpha Rabbit Monoclonal antibody for IF on cajal-retzius cell fetal brain cortex in a previous project. I am using rat, and We intend to use the antibody for IP next. We are interested in examining cajal-retzius cell fetal brain cortex as well as renal cell carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IP?

E. Krishna

Verified customer

Asked: 2013-09-11

Answer

I viewed the website and datasheets of our anti-Hsp90 alpha Rabbit Monoclonal antibody and I see that M01103-3 has been tested on rat in both IF and IP. Thus M01103-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-09-11

Order DetailsPrice
M01103-3

100μL

$370
M01103-3-30ul

30μL sample

$99
M01103-3-carrier-free-1ml

Carrier Free

$2590

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M01103-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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