Product Info Summary
SKU: | M01103-2 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Hsp90 alpha + beta HSP90AA1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01103-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Hsp90 alpha + beta HSP90AA1 Rabbit Monoclonal Antibody catalog # M01103-2. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hsp90 alpha + beta HSP90AA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01103-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EBD-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Hsp90 alpha + beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01103-2 is reactive to HSP90AA1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
80-100 kDa
Calculated molecular weight
84660 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01103-2 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:100
Positive Control
WB: human 293T whole cell, human U251 whole cell, human SiHa whole cell, rat brain tissue, mouse brain tissue, mouse kidney tissue
IHC: rat brain tissue, human brest cancer tissue, human colorectal adenocarcinoma tissue, human lung cancer tissue, human lymphoma tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Hsp90 using anti-Hsp90 antibody (M01103-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp90 antigen affinity purified monoclonal antibody (Catalog # M01103-2) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp90 at approximately 80-100 kDa. The expected band size for Hsp90 is at 85 kDa.
Click image to see more details
Figure 2. IHC analysis of Hsp90 using anti-Hsp90 antibody (M01103-2).
Hsp90 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Hsp90 Antibody (M01103-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Hsp90 using anti-Hsp90 antibody (M01103-2).
Hsp90 was detected in a paraffin-embedded section of human brest cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Hsp90 Antibody (M01103-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Hsp90 using anti-Hsp90 antibody (M01103-2).
Hsp90 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Hsp90 Antibody (M01103-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Hsp90 using anti-Hsp90 antibody (M01103-2).
Hsp90 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Hsp90 Antibody (M01103-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of Hsp90 using anti-Hsp90 antibody (M01103-2).
Hsp90 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Hsp90 Antibody (M01103-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of Hsp90 using anti-Hsp90 antibody (M01103-2).
Hsp90 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Hsp90 Antibody (M01103-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Immunofluorescent analysis of Hela cells, using Hsp90 Antibody.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For HSP90AA1 (Source: Uniprot.org, NCBI)
Gene Name
HSP90AA1
Full Name
Heat shock protein HSP 90-alpha
Weight
84660 MW
Superfamily
heat shock protein 90 family
Alternative Names
Heat shock protein HSP 90-alpha;Heat shock 86 kDa;HSP 86;HSP86;Lipopolysaccharide-associated protein 2;LAP-2;LPS-associated protein 2;Renal carcinoma antigen NY-REN-38;HSP90AA1;HSP90A, HSPC1, HSPCA; HSP90AA1 EL52, HEL-S-65p, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp103, Hsp89, Hsp90, LAP-2, LAP2 heat shock protein 90 alpha family class A member 1 heat shock protein HSP 90-alpha|HSP 86|LPS-associated protein 2|epididymis luminal secretory protein 52|epididymis secretory sperm binding protein Li 65p|heat shock 86 kDa|heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|heat shock 90kD protein, alpha-like 4|heat shock 90kDa protein 1, alpha|heat shock protein 90kDa alpha (cytosolic), class A member 1|heat shock protein 90kDa alpha family class A member 1|lipopolysaccharide-associated protein 2|renal carcinoma NY-REN-38
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSP90AA1, check out the HSP90AA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSP90AA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hsp90 alpha + beta HSP90AA1 Rabbit Monoclonal Antibody (M01103-2)
Hello CJ!
M01103-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiang Q, Wang Y, Li T, Shi K, Li Z, Ma Y, Li F, Luo H, Yang Y, Xu C. Mol Biol Cell. 2011 Apr 15;22(8):1167-80. Doi: 10.1091/Mbc.E10-10-0860. Epub 2011 Feb 23. Heat Shock Protein 90-Mediated Inactivation Of Nuclear Factor-??b Switches Autophagy To ...
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Customer Q&As
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16 Customer Q&As for Anti-Hsp90 alpha + beta HSP90AA1 Rabbit Monoclonal Antibody
Question
We have been able to see staining in human peripheral blood lymphocyte. Do you have any suggestions? Is anti-Hsp90 alpha + beta Rabbit Monoclonal antibody supposed to stain peripheral blood lymphocyte positively?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
According to literature peripheral blood lymphocyte does express HSP90AA1. According to Uniprot.org, HSP90AA1 is expressed in middle temporal gyrus, peripheral blood lymphocyte, placenta, placenta teratocarcinoma, brain, cajal-retzius cell fetal brain cortex, heart, renal cell carcinoma, lymphoblast, cervix carcinoma, pituitary, t-cell, platelet, liver, leukemic t-cell, melanoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have HSP90AA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 2492519, 10409742
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 18318008, 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Peripheral blood lymphocyte, Pubmed ID: 2780322
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 2527334, 15489334
Placenta, and Teratocarcinoma, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Renal cell carcinoma, Pubmed ID: 10508479, 11274138
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2020-01-21
Question
My question regarding product M01103-2, anti-Hsp90 alpha + beta Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01103-2 anti-Hsp90 alpha + beta Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-09
Question
Please see the WB image, lot number and protocol we used for heart using anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-07
Question
I was wanting to use your anti-Hsp90 alpha + beta Rabbit Monoclonal antibody for WB for rat heart on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat heart identification?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
As indicated on the product datasheet, M01103-2 anti-Hsp90 alpha + beta Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-25
Question
Is a blocking peptide available for product anti-Hsp90 alpha + beta Rabbit Monoclonal antibody (M01103-2)?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
We do provide the blocking peptide for product anti-Hsp90 alpha + beta Rabbit Monoclonal antibody (M01103-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-24
Question
We are currently using anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The anti-Hsp90 alpha + beta Rabbit Monoclonal antibody (M01103-2) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-28
Question
Would anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2 work for WB with heart?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
According to the expression profile of heart, HSP90AA1 is highly expressed in heart. So, it is likely that anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2 will work for WB with heart.
Boster Scientific Support
Answered: 2019-07-29
Question
Does anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2 work on canine IHC with heart?
F. Wu
Verified customer
Asked: 2019-05-20
Answer
Our lab technicians have not tested anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine heart in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-20
Question
Is this M01103-2 anti-Hsp90 alpha + beta Rabbit Monoclonal antibody reactive to the isotypes of HSP90AA1?
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
The immunogen of M01103-2 anti-Hsp90 alpha + beta Rabbit Monoclonal antibody is A synthesized peptide derived from human Hsp90 alpha + beta. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-27
Question
Does M01103-2 anti-Hsp90 alpha + beta Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Zhao
Verified customer
Asked: 2018-05-07
Answer
You can see on the product datasheet, M01103-2 anti-Hsp90 alpha + beta Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-07
Question
I see that the anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-16
Question
Is there a BSA free version of anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2 available?
P. Bhatt
Verified customer
Asked: 2018-01-08
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-08
Question
Our lab want to know about to test anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2 on rat heart for research purposes, then I may be interested in using anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
The products we sell, including anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-27
Question
We were satisfied with the WB result of your anti-Hsp90 alpha + beta Rabbit Monoclonal antibody. However we have been able to see positive staining in peripheral blood lymphocyte nucleus. using this antibody. Is that expected? Could you tell me where is HSP90AA1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
According to literature, peripheral blood lymphocyte does express HSP90AA1. Generally HSP90AA1 expresses in nucleus. Regarding which tissues have HSP90AA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 2492519, 10409742
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 18318008, 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Peripheral blood lymphocyte, Pubmed ID: 2780322
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 2527334, 15489334
Placenta, and Teratocarcinoma, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Renal cell carcinoma, Pubmed ID: 10508479, 11274138
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2017-06-28
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-Hsp90 alpha + beta Rabbit Monoclonal antibody M01103-2. Let me know if you need anything else.
M. Anderson
Verified customer
Asked: 2016-12-20
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-20
Question
We ordered your anti-Hsp90 alpha + beta Rabbit Monoclonal antibody for IF on lymphoblast in the past. I am using mouse, and I plan to use the antibody for WB next. My lab would like examining lymphoblast as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
A. Moore
Verified customer
Asked: 2013-03-12
Answer
I have checked the website and datasheets of our anti-Hsp90 alpha + beta Rabbit Monoclonal antibody and it appears that M01103-2 has been tested on mouse in both IF and WB. Thus M01103-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-03-12