Click image to see more details
Product Info Summary
| SKU: | A09630 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-HSP7E HSPA14 Antibody
SKU/Catalog Number
A09630
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HSP7E HSPA14 Antibody (Catalog# A09630). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP7E HSPA14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09630)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human HSPA14. Immunogen sequence location: 421-470
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09630 is reactive to Hspa14 in Human, Mouse, Rat
Applications
A09630 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
54794 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from LOVO cells and NIH/3T3 cells, using HSP7E Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For Hspa14 (Source: Uniprot.org, NCBI)
Gene Name
Hspa14
Full Name
Heat shock 70 kDa protein 14
Weight
54794 MW
Superfamily
heat shock protein 70 family
Alternative Names
heat shock 70kDa protein 14; HSP70-4; HSP70L1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Hspa14, check out the Hspa14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Hspa14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSP7E HSPA14 Antibody (A09630)
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No publications found for A09630
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-HSP7E HSPA14 Antibody
Question
Would A09630 anti-HSP7E antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
You can see on the product datasheet, A09630 anti-HSP7E antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-19
Question
I see that the anti-HSP7E antibody A09630 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-06
Question
Will anti-HSP7E antibody A09630 work on zebrafish WB with secondary oocyte?
Verified Customer
Verified customer
Asked: 2018-02-27
Answer
Our lab technicians have not tested anti-HSP7E antibody A09630 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-HSP7E antibody A09630 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish secondary oocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-27
Question
I was wanting to use your anti-HSP7E antibody for WB for mouse testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse testis identification?
Verified Customer
Verified customer
Asked: 2017-08-03
Answer
You can see on the product datasheet, A09630 anti-HSP7E antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-HSP7E antibody A09630. Let me know if you need anything else.
N. Parker
Verified customer
Asked: 2014-06-30
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-06-30
