Click image to see more details
Product Info Summary
| SKU: | A04286 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-HSP70 HSPA1L Monoclonal Antibody
SKU/Catalog Number
A04286
Size
100μl
Form
Liquid
Description
Boster Bio Anti-HSP70 HSPA1L Monoclonal Antibody catalog # A04286. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP70 HSPA1L Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04286)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
3G1
Isotype
IgG1
Immunogen
Synthetic Peptide of HSP70
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04286 is reactive to HSPA1L in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
70375 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04286 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-2000
IF, 1:100-200
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of Pig Skeletal Muscle with HSP70 Monoclonal antibody diluted at 1:2000.
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Lung Carcicnoma using Mouse Monoclonal antibody diluted at 1:500.
Click image to see more details
Western Blot (WB) analysis of 1) HeLa, 2) Mouse Brain, diluted at 1:2000.
Click image to see more details
Immunofluorescence (IF) analysis of HeLa with antibody (Left) and DAPI (Right) diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For HSPA1L (Source: Uniprot.org, NCBI)
Gene Name
HSPA1L
Full Name
Heat shock 70 kDa protein 1-like
Weight
70375 MW
Superfamily
heat shock protein 70 family
Alternative Names
Heat shock 70 kDa protein 1-like;Heat shock 70 kDa protein 1L;Heat shock 70 kDa protein 1-Hom;HSP70-Hom;HSPA1L; HSPA1L HSP70-1L, HSP70-HOM, HSP70T, hum70t heat shock protein family A (Hsp70) member 1 like heat shock 70 kDa protein 1-like|epididymis secretory sperm binding protein|heat shock 10kDa protein 1-like|heat shock 70 kDa protein 1-Hom|heat shock 70 kDa protein 1L|heat shock 70kD protein-like 1|heat shock 70kDa protein 1-like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPA1L, check out the HSPA1L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPA1L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSP70 HSPA1L Monoclonal Antibody (A04286)
Hello CJ!
A04286 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang,D.,Ripley-Gonzalez,J.W.& Hu,Y.Aerobic Physical Training Protects the Rat Brain Against Exercise-Heat Related Oxidative Damage through the Increased Expression of HSP70.Neurophysiology 51,66–71(2019).https://doi.org/10.1007/s11062-019-09794-9
Species: Rat
A04286 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:400
Purification of heat shock protein 70-associated tumor peptides and its antitumor immunity on hepatoma in mice
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-HSP70 HSPA1L Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-HSP70 HSPA1L Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-HSP70 HSPA1L Monoclonal Antibody
Question
Would anti-HSP70 Monoclonal antibody A04286 work on monkey IF with sperm?
D. Huang
Verified customer
Asked: 2020-04-24
Answer
Our lab technicians have not tested anti-HSP70 Monoclonal antibody A04286 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-HSP70 Monoclonal antibody A04286 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey sperm in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-24
Question
Is this A04286 anti-HSP70 Monoclonal antibody reactive to the isotypes of HSPA1L?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The immunogen of A04286 anti-HSP70 Monoclonal antibody is Synthetic Peptide. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for sperm using anti-HSP70 Monoclonal antibody A04286. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-09
Question
Do you have a BSA free version of anti-HSP70 Monoclonal antibody A04286 available?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HSP70 Monoclonal antibody A04286 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-18
Question
Will A04286 anti-HSP70 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
You can see on the product datasheet, A04286 anti-HSP70 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-17
Question
See below the WB image, lot number and protocol we used for sperm using anti-HSP70 Monoclonal antibody A04286. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-04-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-30
Question
We are currently using anti-HSP70 Monoclonal antibody A04286 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-08
Answer
The anti-HSP70 Monoclonal antibody (A04286) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-08
Question
My question regarding product A04286, anti-HSP70 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04286 anti-HSP70 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-07
Question
I was wanting to use your anti-HSP70 Monoclonal antibody for WB for human sperm on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human sperm identification?
S. Lewis
Verified customer
Asked: 2017-11-03
Answer
As indicated on the product datasheet, A04286 anti-HSP70 Monoclonal antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human sperm in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-03
Question
I see that the anti-HSP70 Monoclonal antibody A04286 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-08-16
Question
Is a blocking peptide available for product anti-HSP70 Monoclonal antibody (A04286)?
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
We do provide the blocking peptide for product anti-HSP70 Monoclonal antibody (A04286). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-07-26
Question
I am interested in to test anti-HSP70 Monoclonal antibody A04286 on human sperm for research purposes, then I may be interested in using anti-HSP70 Monoclonal antibody A04286 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Patel
Verified customer
Asked: 2015-01-06
Answer
The products we sell, including anti-HSP70 Monoclonal antibody A04286, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-01-06
Question
Would anti-HSP70 Monoclonal antibody A04286 work for WB with sperm?
J. Jones
Verified customer
Asked: 2013-07-16
Answer
According to the expression profile of sperm, HSPA1L is highly expressed in sperm. So, it is likely that anti-HSP70 Monoclonal antibody A04286 will work for WB with sperm.
Boster Scientific Support
Answered: 2013-07-16
