Product Info Summary
SKU: | M01280-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Hsp60 HSPD1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01280-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Hsp60 HSPD1 Rabbit Monoclonal Antibody catalog # M01280-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hsp60 HSPD1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01280-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DOI-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Hsp60
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01280-1 is reactive to HSPD1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
61055 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01280-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
IHC: human kidney tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Western blot analysis of Hsp60 expression in (1) HeLa cell lysate; (2) 293T cell lysate; (3) NIH/3T3 cell lysate; (4) Mouse heart lysate; (5) PC-12 cell lysate; (6) Rat heart lysate.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human kidney, using Hsp60 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For HSPD1 (Source: Uniprot.org, NCBI)
Gene Name
HSPD1
Full Name
60 kDa heat shock protein, mitochondrial
Weight
61055 MW
Superfamily
chaperonin (HSP60) family
Alternative Names
60 kDa heat shock protein, mitochondrial;60 kDa chaperonin;Chaperonin 60;CPN60;Heat shock protein 60;HSP-60;Hsp60;HuCHA60;Mitochondrial matrix protein P1;P60 lymphocyte protein;HSPD1;HSP60; HSPD1 CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13 heat shock protein family D (Hsp60) member 1 60 kDa heat shock protein, mitochondrial|60 kDa chaperonin|P60 lymphocyte protein|chaperonin 60|epididymis secretory sperm binding protein|heat shock 60kDa protein 1 (chaperonin)|heat shock protein 65|mitochondrial matrix protein P1|short heat shock protein 60 Hsp60s1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPD1, check out the HSPD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hsp60 HSPD1 Rabbit Monoclonal Antibody (M01280-1)
Hello CJ!
M01280-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression and location of HSP60 and HSP10 in the heart tissue of heat-stressed rats
Hepatic mitochondrial and ER stress induced by defective PPAR? signaling in the pathogenesis of hepatic steatosis
Hager L, Li L, Pun H, Liu L, Hossain Ma, Maguire Gf, Naples M, Baker C, Magomedova L, Tam J, Adeli K, Cummins Cl, Connelly Pw, Ng Ds. J Biol Chem. 2012 Jun 8;287(24):20755-68. Doi: 10.1074/Jbc.M112.340919. Epub 2012 Apr 12. Lecithin:Cholesterol Ac...
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